EasyProbe™ Propidium Iodide Dead Cell Stain

EasyProbes™ Propidium iodide is a commonly used dead-cell stain that emits red fluorescence upon binding to DNA. Our EasyProbes™ Propidium Iodide Dead Cell Stain is formulated in a ready-to-use solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells. Propidium iodide is membrane-impermeant and excluded from viable cells. It is therefore commonly used for identifying dead cells in a population, and for counting dead cells, measuring apoptosis, and sorting cells based on DNA content.

Features

Suggestions for use

• In most cases 2 drops/1×106 cells and an incubation of 15 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases simply add more, or fewer drops until the optimal staining intensity is obtained.
• EasyProbes™ Propidium Iodide Dead Cell Stain is excited with a xenon or mercury-arc lamp or with the 488 line of an argon-ion laser when bound to DNA, with an emission maximum at 617 nm. Generally, propidium iodide fluorescence is detected in the FL2 channel of flow cytometers.

Specifications:
Excitation/Emission:	535/617 nm
Shipping Condition:	Ambient
Storage Conditions:	4ºC, protect from light
Unit Size:	10 mL