NucTracer 21 Green Nuclear Stain

NucTracer 21 Green Nuclear Stain (Known as SYTO 21 Green Nucleic Acid Stain, Trademark of Thermo Fisher Scientific) is a cell-permeant nucleic acid stain that shows a large fluorescence enhancement upon binding nucleic acids. The NucTracer 21 dye can be used to stain RNA and DNA in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with NucTracer 21 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining. This dye is provided as 5 mM solution in DMSO.

Features:
 

• Permeability to virtually all cell membranes, including mammalian cells and bacteria.
• High molar absorptivity, with extinction coefficients >50,000 cm^-1 M^-1 at visible absorption maxima.
• Extremely low intrinsic fluorescence, with quantum yields typically <0.01 when not bound to nucleic acids.
• Quantum yields that are typically >0.4 when bound to nucleic acids.