MitoTracer™ Deep Red FM | ABP Biosciences

Introduction

MitoTracer™ Deep Red FM (Known as MitoTracker® Deep Red FM, Trademark of Thermo Fisher Scientific) is a Near IR-fluorescent dye that stains mitochondria in live cells and its accumulation is dependent upon membrane potential. The dye is well-retained after aldehyde fixation.

Specifications:
Excitation/Emission:	645/665 nm
Shipping Condition:	Ambient
Storage Conditions:	-20ºC, protect from light
Molecular Formula:	C₃₄H₃₆Cl₂N₂
Molecular Weight:	543.58
CAS Number:	–

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Documents

Protocol (PDF):	C043
MSDS (PDF):	MSDS-C043
COA (PDF):	C043

Reference:

Dysfunctions of cellular oxidative metabolism in patients with mutations in the NDUFS1 and NDUFS4 genes of complex I.

Iuso A, Scacco S, Piccoli C, Bellomo F, Petruzzella V, Trentadue R, Minuto M, Ripoli M, Capitanio N, Zeviani M, Papa S
J Biol Chem (2006) 281:10374-10380

Control of mitochondrial structure and function by the Yorkie/YAP oncogenic pathway.

Nagaraj R, Gururaja-Rao S, Jones KT, Slattery M, Negre N, Braas D, Christofk H, White KP, Mann R, Banerjee U,
Genes Dev (2012) 26:2027-2037
Product usage: Proliferation, DNA Synthesis, Click-iT EdU, Imaging, in vivo

Central role of mitofusin 2 in autophagosome-lysosome fusion in cardiomyocytes.

Zhao T, Huang X, Han L, Wang X, Cheng H, Zhao Y, Chen Q, Chen J, Cheng H, Xiao R, Zheng M,
J Biol Chem (2012) 287:23615-23625
Product usage: Lysotracker, ER-Tracker, Mitotracker, Autophagy, Imaging

Frequently asked questions (FAQs)

It looks like my Mitotracker™ dye is staining more than just the mitochondria. Why?
This is typically a result of using too high of a concentration of the MitoViewr™ dye. Most organic dyes are used in the low micromolar range. The MitoView™ dyes are used at a much lower concentration, around 50–200 nanomolar. Higher concentrations can cause background fluorescence and non-mitochondrial staining.

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C043	MitoTracer™ Deep Red FM	645/665	1 mg	$130