Introduction
DRAQ5™ Fluorescent Probe is a far-red DNA stain for live or fixed cells and easy multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins.
DRAQ5™ Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. Because of its far-red excitation and emission, the DRAQ5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ5 Fluorescent Probe is compatible with many existing protocols across a wide range of instrumentation platforms.
Specifications:
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Excitation/Emission:
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647/680 nm
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Shipping Condition:
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Ambient
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Storage Conditions:
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2-8ºC, protect from light
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Molecular Formula:
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C22H28N4O4
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Molecular Weight:
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412.49
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CAS Number:
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254098-36-7
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Documents
Reference:
A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometryAn automated image capture and quantitation approach to identify proteins affecting tumor cell proliferation.
Smith, P.J., et al.
J Immunol Methods (1999) 229(1-2):131-9
A novel deep red/low infrared fluorescent flow cytometric probe, DRAQ5, for the discrimination of intact nucleated cells in apoptotic cell populationsDetection of pyruvate dehydrogenase E1 alpha-subunit deficiencies in females by immunohistochemical demonstration of mosaicism in cultured fibroblasts.
Wiltshire, M., et al.
Cytometry (2000) 39(3):217-23Product usage:
Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopyLoss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.
Smith, P.J., et al.
Cytometry (2000) 40(4):280-91