EasyMag DNA Methylation Kit

Introduction

EasyMag DNA Methylation Kit integrates DNA denaturation and bisulfite conversion processes into one-step coupled to a magnetic bead based clean-up for high-throughput methylation analysis. Also, the kit has been streamlined for high yield recovery of DNA following DNA bisulfite conversion. Desulphonation and clean-up of the converted DNA is performed while bound to the MagPure Beads. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.

Features

Complete, high-throughput, bisulfite conversion of DNA in less than 2 hours.
A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.

Technical Specifications

Applications	Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion	>99%
Elution Volume	≥25 μL
Input	500 pg – 2 μg of DNA
Process Time	2 hours
Recovery	>70%
Sample Source	Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

To Order

Documents

Protocol (PDF):	D201
MSDS (PDF):	CT Conversion Reagent	Dilution Buffer
	Dissolving Buffer	Binding Buffer
	Wash Buffer	Desulphonation Buffer
	Elution Buffer

Related Products

Buy	Cat.No.	Product Name	Unit Size	Price
	D201-1	EasyMag DNA Methylation Kit	96 preps	$200
	D201-2	EasyMag DNA Methylation Kit	4×96 preps	$600