Home » Product » EasyMag DNA Methylation Kit

 

EasyMag DNA Methylation Kit

Introduction

EasyMag DNA Methylation Kit integrates DNA denaturation and bisulfite conversion processes into one-step coupled to a magnetic bead based clean-up for high-throughput methylation analysis. Also, the kit has been streamlined for high yield recovery of DNA following DNA bisulfite conversion. Desulphonation and clean-up of the converted DNA is performed while bound to the MagPure Beads. The kit is optimized to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including endonuclease digestion, sequencing, microarrays, etc.


Features

  • Complete, high-throughput, bisulfite conversion of DNA in less than 2 hours.
  • A coupled heat denaturation/conversion reaction step streamlines the conversion of non-methylated cytosines into uracil.
  • High throughput (96-well), automated desulphonation and recovery of bisulfite-treated DNA.
  • Recovered DNA is ideal for downstream analyses such as PCR, endonuclease digestion, sequencing, microarrays, etc.


Technical Specifications

Applications Purified, converted DNA is of high-quality and well-suited for downstream processes, including library preparation for Next-generation sequencing, PCR amplification, etc.
Conversion >99%
Elution Volume ≥25 μL
Input 500 pg – 2 μg of DNA
Process Time 2 hours
Recovery >70%
Sample Source Purified genomic DNA, endonuclease-digested DNA, linearized plasmid DNA, etc. DNA should be high-quality and RNA-free.

 

To Order

Buy Cat.No. Product Name Unit Size Price
D201-1 EasyMag DNA Methylation Kit 96 preps $180
D201-2 EasyMag DNA Methylation Kit 4×96 preps $560

Documents

Protocol (PDF): D201
MSDS (PDF):