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Biotin Derivatives

Introduction

The interaction between biotin and avidin or streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the avidin-biotin interaction to be used for the development of robust and highly sensitive assays. The avidin-biotin detection system allows an almost unlimited number of primary detection reagents (i.e., antibodies, nucleic acid probes and ligands) to be easily captured, immobilized or detected with secondary detection reagents generated by modifying avidin or streptavidin.

Biotin Derivatives are employed in biotechnology as biotinylation reagents. ABP Biosciences offers many biotin reagents to facilitate biotinylation in the lab.


Figure 1. Application of biotin derivatives

Biotin Derivative Selection Guide

Cat. No. Product Name Application Unit Size
C403 Biotin-11-dUTP, 1 mM in TE buffer TUNEL, FISH 50 µL
C404 Biotin-11-UTP, 1 mM in TE buffer TUNEL, FISH 50 µL
A031 Biotin-X Annexin V Apoptosis 25 µg
C303 Biotin Alkyne Click labeling 5 mg
C304 Biotin Azide Click labeling 5 mg
C330 Biotin DBCO Click labeling 1 mg
B003 Biotin, SE Biotinylation reagent 100 mg
B004 Biotin-X, SE Biotinylation reagent 100 mg
B005 Biotin-XX, SE Biotinylation reagent 50 mg
B006 Biotin-PEG3, SE Biotinylation reagent 25 mg

To order

Buy Cat.No. Product name Ex/Em (nm) Size Price
B003 Biotin, SE 100 mg $80
B004 Biotin-X, SE 100 mg $120
B005 Biotin-XX, SE 50 mg $160
B006 Biotin-PEG3, SE 25 mg $160