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Biotin Azide

Introduction

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The biotin azide is reactive with terminal alkyne via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Features

  • Efficiency—the click reaction is complete in less than 1 hour;
  • Specificity—the reaction between the label and detection tag is selective and specific;
  • Stability—the reaction product contains an irreversible, covalent bond;
  • Biologically inert—the components of the reaction do not undergo any side reactions.

 
Figure 1. Click chemistry labeling

Specifications

Label: Biotin
Ex/Em:
Detection Method:
Solubility: DMSO, DMF
Molecular Weight: 400.50
Product Size: 5 mg
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Click chemistry labeling

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C304 Biotin Azide 5 mg $90
 

Documents

Datasheet (PDF): C304
MSDS (PDF): C304

Reference

Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,
J Am Chem Soc (2008) 130:11576-11577

Robust fluorescent detection of protein fatty-acylation with chemical reporters.
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,
J Am Chem Soc (2009) 131:4967-4975

Vila A, Tallman KA, Jacobs AT, Liebler DC, Porter NA, Marnett LJ,
Chem Res Toxicol (2008) 21:432-444
Alkynyl sugar analogs for the labeling and visualization of glycoconjugates in cells.
Hsu TL, Hanson SR, Kishikawa K, Wang SK, Sawa M, Wong CH
Proc Natl Acad Sci U S A (2007) 104:2614-2619

Metabolic labeling of sialic acids in living animals with alkynyl sugars.
Chang PV, Chen X, Smyrniotis C, Xenakis A, Hu T, Bertozzi CR, Wu P
Angew Chem Int Ed Engl (2009) 48:4030-4033

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