Introduction
Features
Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The biotin alkyne is reactive with azide via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.
Features
- Efficiency—the click reaction is complete in less than 1 hour;
- Specificity—the reaction between the label and detection tag is selective and specific;
- Stability—the reaction product contains an irreversible, covalent bond;
- Biologically inert—the components of the reaction do not undergo any side reactions.
Figure 1. Click chemistry labeling
Specifications
| Label: | Biotin |
|
| Ex/Em: | – | |
| Detection Method: | – | |
| Solubility: | DMSO, DMF | |
| Molecular Weight: | 413.53 | |
| Product Size: | 5 mg | |
| Storage Conditions: | -20 ℃, protect from light | |
| Shipping Condition: | Room Temperature | |
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Applications Click chemistry labeling |
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To order
Documents
| Datasheet (PDF): | C303 |
| MSDS (PDF): | C303 |
Reference
Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,
J Am Chem Soc (2008) 130:11576-11577
J Am Chem Soc (2008) 130:11576-11577
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,
J Am Chem Soc (2009) 131:4967-4975
J Am Chem Soc (2009) 131:4967-4975
Dias WB, Cheung WD, Wang Z, Hart GW,
J Biol Chem (2009) 284:21327-21337
J Biol Chem (2009) 284:21327-21337
