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Biotin DBCO

Introduction

Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. DBCO alkynes can be used to perform click reactions with azide-modified targets without the use of heavy metal catalysis. DBCO reactions are ideal for surface labeling of live cells and also minimize damage to fluorescent proteins like GFP or R-PE.
The biotin DBCO is reactive with azide via a Strain-promoted Azide-Alkyne Click Chemistry reaction (SPAAC). Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.

Features

  • Efficiency—the click reaction is complete in less than 1 hour;
  • Specificity—the reaction between the label and detection tag is selective and specific;
  • Stability—the reaction product contains an irreversible, covalent bond;
  • Biologically inert—the components of the reaction do not undergo any side reactions.

 
Figure 1. Click chemistry labeling

Specifications

Label: Biotin
Ex/Em:
Detection Method:
Solubility: DMSO, DMF
Molecular Weight: 747.95
Product Size: 1 mg
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Click chemistry labeling

 

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C330 Biotin DBCO 1 mg $180
 

Documents

Datasheet (PDF): C330
MSDS (PDF): C330

Reference

Click-mediated labeling of bacterial membranes through metabolic modification of the lipopolysaccharide inner core.
Dumont A, Malleron A, Awwad M, Dukan S, Vauzeilles B,
Angew Chem Int Ed Engl (2012) 51:3143-3146

Winz ML, Samanta A, Benzinger D, Jäschke A,
Nucleic Acids Res (2012) 40:e78-e78

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