JC-1 Dye | ABP Biosciences

Introduction

JC-1 Dye (5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine chloride) is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. JC-1 exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that exhibit a broad excitation spectrum and an emission maximum at ~590 nm. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential. JC-1 is particularly useful for apoptosis studies. In apoptotic cells, the dye stays in the cytoplasm and fluoresces green.

Specifications:
Excitation/Emission:	514/529 nm; 585/590 nm
Shipping Condition:	Ambient
Storage Conditions:	2-8ºC, protect from light
Molecular Formula:	C₂₅H₂₇Cl₄IN₄
Molecular Weight:	652.23
CAS Number:	47729-63-5

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Documents

Protocol (PDF):	C045
MSDS (PDF):	MSDS-C045
COA (PDF):	C045

Reference

A water soluble CoQ10 formulation improves intracellular distribution and promotes mitochondrial respiration in cultured cells.

Bergamini C, Moruzzi N, Sblendido A, Lenaz G, Fato R,
PLoS One (2012) 7:e33712-e33712

SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells.

Liu C, Ogando D, Bonanno JA,
Mol Vis (2011) 17:2473-2481

Saikosaponin-d Enhances the Anticancer Potency of TNF-a via Overcoming Its Undesirable Response of Activating NF-Kappa B Signalling in Cancer Cells.

Wong VK, Zhang MM, Zhou H, Lam KY, Chan PL, Law CK, Yue PY, Liu L,
Evid Based Complement Alternat Med (2013) 2013:745295-745295

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C045	JC-1 Dye	514/529; 585/590	5 mg	$195