CFDA | ABP Biosciences

Introduction

5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. As compared with fluorescein, carboxyfluorescein contains extra negative charges and is therefore better retained in cells.

Specifications:
Excitation/Emission:	490/520 (after cleavage)
Shipping Condition:	Ambient
Storage Conditions:	-20ºC, protect from light
Molecular Formula:	C25H16O9
Molecular Weight:	460.40
CAS Number:	124387-19-5

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Documents

Protocol (PDF):	C031
MSDS (PDF):	MSDS-C031
COA (PDF):	C031

References:

Nonanoic Acid, a Fungal Self-Inhibitor, Prevents Germination of Rhizopus oligosporus Sporangiospores by Dissipation of the pH Gradient.
Breeuwer P, et al.
Appl Environ Microbiol (1997) 63:178-178

Fluorescent microscopic system for detection of microorganisms and organaic compounds on Mars.

Saito T, Kawasaki Y, Kaneko T, Kobayashi K, Miyakawa A
Proc SPIE-Int Soc Opt Eng (1999) 3755:24-32

Fluorescent dyes for lymphocyte migration and proliferation studies.

Parish CR
Immunol Cell Biol (1999) 77:499-508

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C031	CFDA	490/520	100 mg	$110