5(6)-CDCFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-CDCFDA yields the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pKa of 4.8, this dye can be used as an acidic pH sensor.
Specifications:
Excitation/Emission:
504/529 nm (after cleavage)
Shipping Condition:
Ambient
Storage Conditions:
-20ºC, protect from light
Molecular Formula:
C25H14Cl2O9
Molecular Weight:
529.29
CAS Number:
127770-45-0