Introduction
BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm. This dye is provided as 1 mg solid (C028) and 1 mg/mL solution in DMSO (C029).
Specifications:
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Excitation/Emission:
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490/535 (after cleavage)
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Shipping Condition:
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Ice pack
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Storage Conditions:
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-20ºC, protect from light
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Molecular Formula:
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N/A
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Molecular Weight:
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N/A
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CAS Number:
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117464-70-7
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Documents
Reference:
Jayadev S, Petranka JG, Cheran SK, Biermann JA, Barrett JC, Murphy E
J Biol Chem (1999) 274:8261-8268
Product usage:
Parish CR
Immunol Cell Biol (1999) 77:499-508
Product usage:
Iizuka T, Tanaka T, Suematsu M, Miura S, Watanabe T, Koike R, Ishimura Y, Ishii H, Miyasaka N, Miyasaka M
J Immunol (2000) 164:2463-2471