Phase Lock Gel, Heavy and Light

Phase Lock Gel (PLG) is a unique product that eliminates interphase-protein contamination during organic extraction and ensures faster results with improved recoveries. Phase Lock Gel, when used during phenol, phenol:chloroform, or phenol:chloroform:isoamyl alcohol extractions, migrates under centrifuge force to form a tight seal between the aqueous and organic phase. The organic phase and the interphase materials are effectively trapped below the Phase Lock Gel barrier, thus enabling complete and easy decanting or pipetting of the entire aqueous phase. The benefits are increased yields of up to 30%, increased protection from exposure to hazardous compounds, and no risk of interphase sample contamination.

Phase Lock Gel is inert, stable to heating and does not interfere with standard nucleic acid restriction and modification enzymes. In fact, many of the reactions can be carried out in the presence of Phase Lock Gel at the appropriate temperature and then terminated by the addition of phenol or phenol:chloroform. The nucleic acid can then be extracted by following standard protocols. Phase Lock Gel can be present during the heat inactivation of enzymes (65°C for 10 minutes) prior to organic extraction.

Features

• The recovery rate of nucleic acid is significantly higher than that of traditional technology.
• Effectively avoid the contamination caused by separating the aqueous phase from the organic phase.
• Experimenters can effectively avoid exposure to toxic organic reagents.
• Can be used together with various phenol chloroform extraction reagents and kits.

Selection Guide