iScript™ IV First-Strand cDNA Synthesis Kit

iScript™ IV Reverse Transcriptase (RT) is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first-strand cDNA synthesis from RNA templates with secondary structures and high GC content. The iScript™ IV Reverse Transcriptase is engineered to work under high temperatures (50-55°C), which can further facilitate to resolve the secondary structures and high GC problems of RNA. Besides, the iScript™ IV RT is significantly improved in inhibitor resistance, processivity, and reaction speed. iScript™ IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples.

iScript™ IV First-Strand cDNA Synthesis Kit is a proprietary mixture of all materials required for first-strand cDNA synthesis. This optimized RT Reaction Mix (2X) contains dNTPs, and a balanced concentration for Oligo(dT)₂₀ and Random Primers. The RT Enzyme Mix contains iScript™ IV Reverse Transcriptase and RNase Inhibitor. Oligo(dT)₂₀ anneals selectively to the poly(A) tail of mRNAs. Random Primers do not require the presence of poly(A) and they are utilized for the transcription of mRNA 5’-end regions. The first-strand cDNA can be directly used as a template in PCR.

Applications

• cDNA synthesis for PCR.
• Construction of cDNA libraries.
• Generation of probes for hybridization.