BCECF | ABP Biosciences

Introduction

BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.

Specifications:
Excitation/Emission:	490/535
Shipping Condition:	Ambient
Storage Conditions:	2-8ºC, protect from light
Molecular Formula:	C27H20O11
Molecular Weight:	520.45
CAS Number:	85138-49-4

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Documents

Protocol (PDF):	C027
MSDS (PDF):	MSDS-C027
MSDS (PDF):	C027

Reference:
Fluorescent indicators for intracellular pH.
Han J, Burgess K,
Chem Rev (2010) 110:2709-2728

Wavelength Considerations in Confocal Microscopy of Botanical Specimens.
Fricker MD, White NS
J Microsc (1992) 166:29-29

Calibration Methods and Avoidance of Errors in Measurement of Intracellular pH (pHcyt) Using the Indicator Bis (Carboxyethyl)-5(6)-Carboxyfluorescein (BCECF) in Human Platelets.
Valant PA, Haynes DH
J Fluorescence (1992) 2:191-191

Related Products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C027	BCECF	490/535	5 mg	$150