iClick™ EdU Andy Fluor™ 488 Imaging Kit

Introduction

Our iClick™ EdU Andy Fluor™ 488 Imaging Kit is a superior alternative to the traditional BrdU cell proliferation assay that is optimized for fluorescence microscopy applications. In this assay the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Andy Fluor™ dye in a fast, highly-specific click reaction. This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

Features

Simple: Labeling is complete in two steps
Efficient: No denaturation steps or harsh treatment required
Content-rich results: Better preservation of cell morphology, antigen structure, and DNA integrity
Consistent: Not dependent on variable antibody lots for detection

Specifications

Platform:	Fluorescence Microscope
Detection Method:	Fluorescent
Ex/Em:	495/520 nm
Product Size:	50 assays

Applications

Cell proliferation analysis in vitro and in vivo.

Figure 1. Detection of cell proliferation in cell. A549 cells were treated with 10 μM EdU for 2 hr, then detected with Andy Fluor™ 488 azide (green), cells were counterstained with DAPI (blue).

Figure 2. Detection of cell proliferation in mouse tissue. A mouse was injected intraperitoneally with 5 mg EdU per kilogram body weight, then sacrificed at 0, 12, and 24 hr. The mouse intestine tissue was detected with Andy Fluor™ 488 azide (green), and counterstained with DAPI (blue).

To Order

Documents

Protocol (PDF):	A003
MSDS (PDF):	Component A Component C Component E	Component B Component D Component F
COA (PDF):	COA-A003

Cited Reference:

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Cells 2020, 9(5), 1069; https://doi.org/10.3390/cells9051069

Synergistic effect of paclitaxel and verapamil to overcome multi-drug resistance in breast cancer cells
Peng Li, Di Zhong, Pei-yao Gong
Biochemical and Biophysical Research Communications, 2019, 516, 183-188, https://doi.org/10.1016/j.bbrc.2019.05.189

Distinct Neutrophil Populations in the Spleen During PICS
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Front. Immunol. 2020, 11:804. doi: 10.3389/fimmu.2020.00804

Chenodeoxycholic acid suppresses AML progression through promoting lipid peroxidation via ROS/p38 MAPK/DGAT1 pathway and inhibiting M2 macrophage polarization
Jinting Liu, Yihong Wei, Wenbo Jia, Can Can, Ruiqing Wang, Xinyu Yang, Chaoyang Gu, Fabao Liu, Chunyan Ji, Daoxin Ma
Redox Biology, 2022, 56, 102452. https://doi.org/10.1016/j.redox.2022.102452

Deregulation of angiopoietin-like 4 slows ovarian cancer progression through vascular endothelial growth factor receptor 2 phosphorylation
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Cancer Cell Int 21, 171 (2021). https://doi.org/10.1186/s12935-021-01865-4

Evaluation of equine xenogeneic mixed lymphocyte reactions using 5-ethynyl-2’-deoxyuridine (EdU)
Kate E. Birdwhistell, David J. Hurley, Bradley Heins, John F. Peroni
Veterinary Immunology and Immunopathology, 2022, 249, 110430, https://doi.org/10.1016/j.vetimm.2022.110430.

Osteochondral Allograft and Xenograft Immunogenicity Decrease Following Ex Vivo Tissue Culture
Kate E. Birdwhistell, Samuel P. Franklin, David J. Hurley, Bradley D. Heins, John F. Peroni
Journal of Cartilage & Joint Preservation, 2023, 100115, https://doi.org/10.1016/j.jcjp.2023.100115.

Production and characterization of avian crypt-villus enteroids and the effect of chemicals
Acharya, M., Arsi, K., Donoghue, A.M. et al.
BMC Vet Res 16, 179 (2020). https://doi.org/10.1186/s12917-020-02397-1

Differential response of macrophages and neutrophils to trypanosome infections in zebrafish: occurrence of foamy macrophages
Sem H. Jacobs, Éva Dóró, Ffion R. Hammond, Mai E. Nguyen-Chi, Georges Lutfalla, Geert F. Wiegertjes, Maria Forlenza
bioRxiv 2020.11.01.363721; doi: https://doi.org/10.1101/2020.11.01.363721

Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis
Xing Wei, Li Zhang, Yiqun Zhang, Cody Cooper, Chris Brewer, Chia-Feng Tsai, Yi-Ting Wang, Micah Glaz, Hunter B. Wessells, Jianwen Que, Mark A. Titus, Vincenzino Cirulli, Adam Glaser, Tao Liu, Nicholas P. Reder, Chad J. Creighton, Li Xin
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SR9009 has REV-ERB–independent effects on cell proliferation and metabolism
Pieterjan Dierickx, Matthew J. Emmett, Chunjie Jiang, and Mitchell A. Lazar
PNAS, 2019, 116(25): 12147-12152. https://doi.org/10.1073/pnas.1904226116

Occurrence of foamy macrophages during the innate response of zebrafish to trypanosome infections
Sem H Jacobs, Eva Dóró, Ffion R Hammond, Mai E Nguyen-Chi, Georges Lutfalla, Geert F Wiegertjes, Maria Forlenza
eLife 2021, 10:e64520. https://doi.org/10.7554/eLife.64520

Related Products

Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A003	iClick™ EdU Andy Fluor™ 488 Imaging Kit	495/520	50 assays	$350