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Cell-Quant™ MTT Cell Proliferation Assay Kit

Introduction

Our Cell-Quant™ MTT Cell Proliferation Assay Kit provides a standard cell proliferation analysis based on cellular metabolic activity. MTT is an widely established reagent that is used to determine cell number in a variety of applications. Live cells reduce MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader. This kit provides a complete package for performing a cell-counting assay that can detect from 2000 to 250,000 cells, depending on the cell type and conditions. The kit provides a sufficient amount of material for ~1000 assays.

 

Specifications

Platform: Microplate Reader
Detection Method: Colorimetric
OD: 570 nm
Product Size: 1000 assays

Figure 1. Quantitation of Jurkat cells using the Cell-Quant™ MTT Cell Proliferation Assay Kit. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated for 4 hr with Cell-Quant™ MTT Cell Proliferation Assay Kit, absorbance was measured at 570 nm.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A015 Cell-Quant™ MTT Cell Proliferation Assay Kit 570/- 1000 assays $180

Documents

Protocol (PDF): A015  
MSDS (PDF):  Component A Component B
COA (PDF): COA-A015  

Reference:

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