Cell-ID™ CFSE Cell Proliferation Kit

Introduction

Our Cell-ID™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.

Features

Superior performance: Bright, single-peak staining enables visualization of multiple generations
Long-term signal stability: Well-retained in cells for several days post stain
Non-toxic: Staining does not adversely effect cell health
Simple, robust staining protocol

Specifications

Platform:	Flow Cytometer
Detection Method:	Fluorescent
Ex/Em:	490/517 nm
Product Size:	1000 assays

Applications

Cell generation analysis by flow cytometry.

Cell generation analysis with Cell-ID™ CFSE Cell Proliferation Kit. Jurkat cells (~1×10⁶ cells/ml) were stained with CFSE dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.

To Order

Documents

Protocol (PDF):	A001
MSDS (PDF):	MSDS-A001
COA (PDF):	COA-A001

Reference:

Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution.

Filby A, Begum J, Jalal M, Day W,
Methods (2015) 82:29-37

Optimized staining and proliferation modeling methods for cell division monitoring using cell tracking dyes.

Tario JD, Humphrey K, Bantly AD, Muirhead KA, Moore JS, Wallace PK,
J Vis Exp (2012) e4287-e4287

Related Products

Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A001	Cell-ID™ CFSE Cell Proliferation Kit	490/517	1000 assays	$180