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Cell-ID™ CFSE Cell Proliferation Kit

Introduction

Our Cell-ID™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.


Features

  • Superior performance: Bright, single-peak staining enables visualization of multiple generations
  • Long-term signal stability: Well-retained in cells for several days post stain
  • Non-toxic: Staining does not adversely effect cell health
  • Simple, robust staining protocol


Specifications

Platform: Flow Cytometer
Detection Method: Fluorescent
Ex/Em: 490/517 nm
Product Size: 1000 assays


Applications

Cell generation analysis by flow cytometry.

product Image
Cell generation analysis with Cell-ID™ CFSE Cell Proliferation Kit. Jurkat cells (~1×106 cells/ml) were stained with CFSE dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A001 Cell-ID™ CFSE Cell Proliferation Kit 490/517 1000 assays $180

Documents

Protocol (PDF): A001
MSDS (PDF):  MSDS-A001
COA (PDF): COA-A001

Reference:

Appraising the suitability of succinimidyl and lipophilic fluorescent dyes to track proliferation in non-quiescent cells by dye dilution.
Filby A, Begum J, Jalal M, Day W,
Methods (2015) 82:29-37

Optimized staining and proliferation modeling methods for cell division monitoring using cell tracking dyes.
Tario JD, Humphrey K, Bantly AD, Muirhead KA, Moore JS, Wallace PK,
J Vis Exp (2012) e4287-e4287