Introduction
Our Cell-ID™ CFSE Cell Proliferation Kit is used for in vitro and in vivo labeling of cells to trace multiple generations using dye dilution by flow cytometry.
Features
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Superior performance: Bright, single-peak staining enables visualization of multiple generations
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Long-term signal stability: Well-retained in cells for several days post stain
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Non-toxic: Staining does not adversely effect cell health
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Simple, robust staining protocol
Specifications
Platform:
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Flow Cytometer
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Detection Method:
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Fluorescent
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Ex/Em:
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490/517 nm
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Product Size:
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1000 assays
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Applications
Cell generation analysis by flow cytometry.
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Cell generation analysis with Cell-ID™ CFSE Cell Proliferation Kit. Jurkat cells (~1×106 cells/ml) were stained with CFSE dye (1 µM) on Day 0. The cells were cultured for 7 days. Fluorescence intensity was measured with FACS flow cytometer.
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Documents
Cited Reference:
ETS1–HMGA2 Axis Promotes Human Limbal Epithelial Stem Cell Proliferation
Bofeng Wang; Huizhen Guo; Dongmei Liu; Siqi Wu; Jiafeng Liu; Xihong Lan; Huaxing Huang; Fengjiao An; Jin Zhu; Jianping Ji; Li Wang; Hong Ouyang; Mingsen Li
Investigative Ophthalmology & Visual Science, 2023, 64, 12. doi:https://doi.org/10.1167/iovs.64.1.12
Epigenetically modulated FOXM1 suppresses dendritic cell maturation in pancreatic cancer and colon cancer
Zhongshi Zhou, Hongdan Chen, Rui Xie, Hongjie Wang, Senlin Li, Qianqian Xu, Na Xu, Qi Cheng, Ying Qian, Rongrong Huang, Zekun Shao, Ming Xiang
Molecular Oncology, 2019, 13, 4, 873-893. https://doi.org/10.1002/1878-0261.12443
Anti-inflammatory and metabolic reprogramming effects of MENK produce antitumor response in CT26 tumor-bearing mice
Yali Tuo, Zijun Zhang, Cheng Tian, Qinyu Hu, Rui Xie, Jian Yang, Hong Zhou, Lili Lu, Ming Xiang
Journal of Leukocyte Biology, Volume 108, Issue 1, Jul 2020, Pages 215–228, https://doi.org/10.1002/JLB.3MA0120-578R