Introduction
eLuminol™ Protein Gel Stain is a high sensitive fluorescent stain designed for detecting proteins in SDS polyacrylamide gels. It is as sensitive as silver stain. Unlike silver stain, however, eLuminol™ Protein Gel Stain has a linear detection range of at least 3 orders of magnitude. Protein gel staining with eLuminol™ is a very simple and fast protocol. It doesn’t require fixation step, only need one simple staining step with 1X eLuminol™ for 90 minutes or less, followed by a simple destaining step. Protein gel staining with eLuminol™ is compatible with downstream protein analyses such as mass spectrometry and Edman-based sequencing.
eLuminol™ Protein Gel Stain is available as a 1,000X concentrated solution, which can be readily diluted to the 1X working solution using methanol/acetic acid/water solution. It is very convenient for shipping and storage. The price of eLuminol™ Protein Gel Stain is comparable to the commercial Coomassie® Blue-based protein gel staining solutions, while eLuminol™ provides far better sensitivity and user-friendliness.
Features
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High sensitivity: ~0.5 ng protein
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Compatibility with standard laboratory equipment
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Wide linear detection range: At least three orders of magnitude
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Compatible with downstream analysis: Compatible with MS and sequencing
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Stable: Stable at room temperature for 1 year
Applications
Protein gel stain
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Comparison eLuminol with SYPRO Ruby
Protocol Step
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SYPRO Ruby
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eLuminol
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Fixation step 1
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15 min, 50% methanol /7.5% acetic acid
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None
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Fixation step 2
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15 min, 50% methanol /7.5% acetic acid
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None
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Staining
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Overnight
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90 min
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Destaining
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30 min, 10% methanol/ 7% acetic acid
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20 min, 10% methanol/ 7% acetic acid
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Rinse step 1
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5 min, water
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Optional: 5 min, water
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Rinse step 2
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5 min, water
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