EasyProbes™ Cell Viability Imaging Kit (Blue/Red)

EasyProbes™ Cell Viability Imaging Kit (Blue/Red) is a ready-to-use assay that can quickly and easily determine the viability of cells. Just add 2 drops each of EasyProbes™ Hoechst 33342 Live Cell Stain reagent and EasyProbes™ Propidium Iodide Dead Cell Stain reagent to 1 mL of cell growth media, then determine viability by counting total vs dead cells. EasyProbes™ Hoechst 33342 reagent stains the nuclei of all the cells and can be detected with a standard DAPI filter. EasyProbes™ PI reagent stains only the nuclei of cells with compromised plasma membrane integrity and is detected using a standard TRITC/RFP (orange) filter set. This kit is appropriate for fluorescence microscopy and flow cytometry.

• EasyProbes™ Hoechst 33342 reagent: stains the nuclei of all cells; detected with a standard DAPI filter (excitation/emission maxima: 350/461 nm)
• EasyProbes™ Propidium Iodide reagent: stains only the nuclei of dead cells with compromised plasma membranes; detected with standard TRITC/RFP (orange) filter set (excitation/emission maxima: 535/617 nm)

Suggestions for use
 

• EasyProbes™ Hoechst 33342 Live Cell Stain reagent and EasyProbes™ Propidium Iodide Dead Cell Stain reagent may be added directly to cells in full growth media or a compatible buffer solution.
• In most cases, 2 drops/mL and an incubation time of 10 to 30 minutes will give bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
   

Specifications:
Detection Method:	Fluorescent
Shipping Condition:	Ambient
Storage Conditions:	2~8ºC, protect from light
Compatible Cells:	Eukaryotic Cells, Mammalian Cells
Product Size:	2 × 10 mL dropper bottles