The Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells provides two-color fluorescence staining on both live (green) and dead (red) bacteria using two probes, SYTO 9 dye and Propidium iodide. SYTO 9 dye is a green-fluorescent nucleic acid dye that stains both live and dead bacteria with intact and damaged cell membranes. Propidium iodide is a red-fluorescent nucleic acid dye that stains only dead bacteria with damaged cell membranes. With an appropriate mixture of SYTO 9 and Propidium iodide, bacteria with intact cell membranes is stained fluorescent green, whereas bacteria with damaged cell membranes is stained fluorescent red. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types.
Features:
- Simplicity: Reagents are simultaneously added, no wash steps are required
- Specificity and reliability: Distinct color for live and dead cell
- Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader
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Specifications |
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| Platform: | Fluorescence Microscopy, Flow Cytometry |
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| Detection Method: | Fluorescent | |
| Ex/Em: | SYTO 9 dye: 480/500; PI: 535/617 nm | |
| Product Size: | 1000 assays | |
| Storage Conditions: | -20 ℃, protect from light | |
| Shipping Condition: | Room temperature | |
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Applications
Cell viability assay |
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Figure 1. Live and dead E.coli cells stained with Cell-Check™ Viability/Cytotoxicity Kit (A018). Live bacteria exhibit green fluorescence, whereas dead bacteria exhibit red fluorescence.
| Protocol (PDF): | A018 | |
| MSDS (PDF): | Component A | Component B |
| COA (PDF): | COA-A018 |
Cited Reference:
Antibacterial effect of bacteriocin XJS01 and its application as antibiofilm agents to treat multidrug-resistant Staphylococcus aureus infection
Yi-Zhou Xiang, Gang Wu, Lin-Yu Yang, Xiao-Jie Yang, Yan-Mei Zhang, Lian-Bing Lin, Xian-Yu Deng, Qi-Lin Zhang
International Journal of Biological Macromolecules, 2022, 196, 13-22, https://doi.org/10.1016/j.ijbiomac.2021.11.136
A novel bacteriocin against Staphylococcus aureus from Lactobacillus paracasei isolated from Yunnan traditional fermented yogurt: Purification, antibacterial characterization, and antibiofilm activity
Yu-Hang Jiang, Wei-Gang Xin, Lin-Yu Yang, Jian-Ping Ying, Zi-Shun Zhao, Lian-Bing Lin, Xiu-Zhang Li, Qi-Lin Zhang
Journal of Dairy Science, 2022, 105(3):2094-2107. https://doi.org/10.3168/jds.2021-21126
Antibacterial activity and action target of phenyllactic acid against Staphylococcus aureus and its application in skim milk and cheese
Yu-Hang Jiang, Jian-Ping Ying, Wei-Gang Xin, Lin-Yu Yang, Xiu-Zhang Li, Qi-Lin Zhang
J. Dairy Sci. 2022, 105:9463–9475. https://doi.org/10.3168/jds.2022-22262
Purification, characterization, and antibacterial and antibiofilm activity of a novel bacteriocin against Salmonella Enteritidis.
Xiang, Y.-Z., Zhang, Y.-M., Liu, Y.-Y., Zhang, M., Lin, L.-B., & Zhang, Q.-L.
Food Control, 2021, 127, 108110. doi:10.1016/j.foodcont.2021.10
A Novel Bacteriocin Against Shigella flexneri From Lactiplantibacillus plantarum Isolated From Tilapia Intestine: Purification, Antibacterial Properties and Antibiofilm Activity.
Jiang Y-H, Xin W-G, Zhang Q-L, Lin L-B and Deng X-Y
Front. Microbiol. 2022, 12:779315. doi: 10.3389/fmicb.2021.779315
Effect of surface sealant on surface roughness and bacterial adhesion of bulk-fill composites
Gunce Ozan, Meltem Mert Eren, Cansu Vatansever and Ugur Erdemir
Polymers and Polymer Composites, 2021, Vol. 29(9S) S475–S484. DOI: 10.1177/09673911211005586
Antibacterial activity and mechanism of action of bacteriocin LFX01 against Staphylococcus aureus and Escherichia coli and its application on pork model
Wei-Gang Xin, Gang Wu, Jian-Ping Ying, Yi-Zhou Xiang, Yu-Hang Jiang, Xian-Yu Deng, Lian-Bing Lin, Qi-Lin Zhang
Meat Science, 2023, 196, 109045, https://doi.org/10.1016/j.meatsci.2022.109045
Streptococcus Mutans adhesion to dental restorative materials after polishing with varioussystems:AConfocal Microscopy study
M.MertErena,G.Ozanb,U.Erdemirb,C.Vatansever
Acta Microscópica, 2021, 30, 1, 53-64
A novel bacteriocin from Lactobacillus salivarius against Staphylococcus aureus: Isolation, purification, identification, antibacterial and antibiofilm activity
Hong-Wei Li, Yi-Zhou Xiang, Man Zhang, Yu-Hang Jiang, Yao Zhang, Ying-Yang Liu, Lian-Bing Lin, Qi-Lin Zhang
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