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Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells

Introduction

The Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells provides two-color fluorescence staining on both live (green) and dead (red) bacteria using two probes, NucView Green and Propidium iodide. NucView Green is a green-fluorescent nucleic acid dye that stains both live and dead bacteria with intact and damaged cell membranes. Propidium iodide is a red-fluorescent nucleic acid dye that stains only dead bacteria with damaged cell membranes. With an appropriate mixture of NucView Green and Propidium iodide, bacteria with intact cell membranes is stained fluorescent green, whereas bacteria with damaged cell membranes is stained fluorescent red. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types.
 

Features:

  • Simplicity: Reagents are simultaneously added, no wash steps are required
  • Specificity and reliability: Distinct color for live and dead cell
  • Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader

Specifications
Platform: Fluorescence Microscopy, Flow Cytometry
 
Detection Method: Fluorescent
Ex/Em: NucView Green: 500/530; PI: 535/617 nm
Product Size: 1000 assays
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room temperature

Applications
Cell viability assay

 


Figure 1. Live and dead E.coli cells stained with Cell-Check™ Viability/Cytotoxicity Kit (A018). Live bacteria exhibit green fluorescence, whereas dead bacteria exhibit red fluorescence.

To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
A018 Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells 1000 assays $180
 

Documents

Protocol (PDF): A018  
MSDS (PDF):  Component A Component B
COA (PDF): COA-A018  

Reference:

Lichens survive in space: results from the 2005 LICHENS experiment.
Sancho LG, de la Torre R, Horneck G, Ascaso C, de Los Rios A, Pintado A, Wierzchos J, Schuster M,
Astrobiology (2007) 7:443-454

Viability of endolithic micro-organisms in rocks from the McMurdo Dry Valleys of Antarctica established by confocal and fluorescence microscopy.
Wierzchos J, De Los Ríos A, Sancho LG, Ascaso C,
J Microsc (2004) 216:57-61

Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.
Kim J, Pitts B, Stewart PS, Camper A, Yoon J,
Antimicrob Agents Chemother (2008) 52:1446-1453