The Cell-Check™ Viability/Cytotoxicity Kit for Bacteria Cells provides two-color fluorescence staining on both live (green) and dead (red) bacteria using two probes, NucView Green and Propidium iodide. NucView Green is a green-fluorescent nucleic acid dye that stains both live and dead bacteria with intact and damaged cell membranes. Propidium iodide is a red-fluorescent nucleic acid dye that stains only dead bacteria with damaged cell membranes. With an appropriate mixture of NucView Green and Propidium iodide, bacteria with intact cell membranes is stained fluorescent green, whereas bacteria with damaged cell membranes is stained fluorescent red. The kit is suitable for use with fluorescence microscopes and flow cytometers. The assay principles are general and applicable to most bacteria types.
- Simplicity: Reagents are simultaneously added, no wash steps are required
- Specificity and reliability: Distinct color for live and dead cell
- Versatility: Suitable for fluorescence microscope, flow cytometer or microplate reader
|Platform:||Fluorescence Microscopy, Flow Cytometry||
|Ex/Em:||NucView Green: 500/530; PI: 535/617 nm|
|Product Size:||1000 assays|
|Storage Conditions:||-20 ℃, protect from light|
|Shipping Condition:||Room temperature|
Cell viability assay
Figure 1. Live and dead E.coli cells stained with Cell-Check™ Viability/Cytotoxicity Kit (A018). Live bacteria exhibit green fluorescence, whereas dead bacteria exhibit red fluorescence.
|MSDS (PDF):||Component A||Component B|
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