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EasyMag FFPE DNA Purification Kit

Introduction

EasyMag FFPE DNA Purification Kit is designed for rapid and reliable isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections. Paraffin removal can be performed using a xylene and ethanol method. Samples are incubated in a specialized lysis buffer along with Proteinase K to reverse crosslinking, effectively releasing short and long DNA fragments. The released DNA is then purified using magnetic bead technology. Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. Purified DNA is suitable for downstream applications including SNP analysis, sequencing, and genotyping.


Features

  • Fast – DNA purification process in less than 20 min post-lysis.
  • Safe – No organic extraction, no ethanol precipitation.
  • High-quality – DNA is suitable for a variety of downstream applications.

 

Technical Specifications

Applications Genomic DNA isolation from formalin-fixed, paraffin-embedded (FFPE) tissue sections.
Sample Source FFPE tissue
Sample Input Up to 25 mg
Elution Volume 50-100 μL
Technology Magnetic Beads
Processing Mode Manual, Automated

 

Figure 1.  Genomic DNA from FFPE samples was isolated using ABP EasyMag FFPE DNA Purification Kit. Eluted DNA was analyzed on a 1% agarose gel. 
 

Table 1. Genomic DNA yield and quality using ABP EasyMag FFPE DNA Purification Kit

To Order

Buy Cat.No. Product Name Unit Size Price
D126-1 EasyMag FFPE DNA Purification Kit 50 preps $130
D126-2 EasyMag FFPE DNA Purification Kit 200 preps $380

Documents

Protocol (PDF):

D126

MSDS (PDF): 

Buffer ATL                Buffer DL

Buffer AW1              Buffer AW2

Buffer AE                 Proteinase K