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Di-8-ANEPPS

Introduction

Di-8-ANEPPS is a fast-response probe that used to measure membrane potential changes. ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. The fast-response probes are operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements.
 

Specifications

Label: Di-8-ANEPPS
Ex/Em: 467/631 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C36H52N2O3S
Molecular Weight: 592.88
CAS Number: 157134-53-7
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Membrane potential indicator

To order

Buy Cat.No. Product name Ex/Em (nm) Size Price
C266 Di-8-ANEPPS 467/631 5 mg $450

 

Documents

Datasheet (PDF): C266
MSDS (PDF): MSDS-C266

References:

Characterization of Potentiometric Membrane Dyes.
Loew LM
Adv Chem Ser (1994) 235:151-151
 
Voltage-Sensitive Dyes and Imaging Neuronal Activity.Voltage-Sensitive Dyes and Imaging Neuronal Activity.
Loew LM
NeuroProtocols (1994) 5:72-72
 
Optical Recording with Single Cell Resolution from a Simple Mammalian Nervous System: Electrical Activity in Ganglia from the Submucous Plexus of the Guinea-Pig Ileum.Optical Recording with Single Cell Resolution from a Simple Mammalian Nervous System: Electrical Activity in Ganglia from the Submucous Plexus of the Guinea-Pig Ileum.
Obaid AL, et al.
Biol Bull (1993) 183:344-344
 

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