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Di-4-ANEPPS is a fast-response probe that used to measure membrane potential changes. ANEP dyes are molecules that fluoresce in response to electrical potential changes in their environment. The fast-response probes are operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements.


Label: Di-4-ANEPPS
Ex/Em: 482/686 nm
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C28H36N2O3S
Molecular Weight: 480.66
CAS Number: 90134-00-2
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Membrane potential indicator

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Buy Cat.No. Product name Ex/Em (nm) Size Price
C265 Di-4-ANEPPS 482/686 5 mg $450



Datasheet (PDF): C265


Monitoring biophysical properties of lipid membranes by environment-sensitive fluorescent probes.
Demchenko AP, Mély Y, Duportail G, Klymchenko AS,
Biophys J (2009) 96:3461-3470
Product usage: Review of the properties and applications of environment-sensitive membrane probes.

External Electric Field-Induced Transmembrane Potentials in Biological Systems: Features, Effects, and Optical Monitoring.
Farkas DL
Electroporation and Electrofusion in Cell Biology, Neumann E, Ed. 1989; (na):na pp. 409-431

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