Cell-Quant™ MTT Cell Proliferation Assay Kit

Introduction

Our Cell-Quant™ MTT Cell Proliferation Assay Kit provides a standard cell proliferation analysis based on cellular metabolic activity. MTT is an widely established reagent that is used to determine cell number in a variety of applications. Live cells reduce MTT to a strongly pigmented formazan product. After solubilization, the absorbance of the formazan can be measured with a microplate absorbance reader. This kit provides a complete package for performing a cell-counting assay that can detect from 2000 to 250,000 cells, depending on the cell type and conditions. The kit provides a sufficient amount of material for ~1000 assays.

Specifications

Platform:	Microplate Reader
Detection Method:	Colorimetric
OD:	570 nm
Product Size:	1000 assays

Figure 1. Quantitation of Jurkat cells using the Cell-Quant™ MTT Cell Proliferation Assay Kit. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated for 4 hr with Cell-Quant™ MTT Cell Proliferation Assay Kit, absorbance was measured at 570 nm.

To Order

Documents

Protocol (PDF):	A015
MSDS (PDF):	Component A	Component B
COA (PDF):	COA-A015

Reference:

Change in colony morphology influences the virulence as well as the biochemical properties of the Mycobacterium avium complex.

Kansal RG, Gomez-Flores R, Mehta RT
Microb Pathog (1998) 25:203-214

Boron trifluoride etherate on silica-A modified Lewis acid reagent (VII). Antitumor activity of cannabigerol against human oral epitheloid carcinoma cells.

Baek SH, Kim YO, Kwag JS, Choi KE, Jung WY, Han DS
Arch Pharm Res (1998) 21:353-356

In situ assessment of cell viability.

Yang H, Acker J, Chen A, McGann L
Cell Transplant (1998) 7:443-451

Related Products

Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A015	Cell-Quant™ MTT Cell Proliferation Assay Kit	570/-	1000 assays	$200