Cell-Quant™ AlamarBlue Cell Viability Reagent

Introduction

Our Cell-Quant™ AlamarBlue Cell Viability Reagent is a redox indicator that yields a colorimetric change and a fluorescent signal in response to metabolic activity. It is a proven safe and non-toxic dye used for quantitative analysis of cell viability and cell proliferation, for cytokine bioassays and in vitro cytotoxicity studies.

Features

Simple workflow: Add, incubate, and read
Flexible: Measure by colorimetric or fluorescent
High sensitivity: Detect as low as 100 cells
Safety: Non-toxic to cells
Low cost and reliable results

Specifications

Platform:	Microplate Reader
Detection Method:	Fluorescent, Colorimetric
Ex/Em:	530-560/590 nm
Product Size:	500 assays, 2500 assays

Applications

Cell proliferation analysis
Cell viability assays, including cytotoxicity
Cytokine assays

Figure 1. Quantitation of Jurkat cells using the Cell-Quant™ AlamarBlue Cell Viability Reagent. Jurkat cells were diluted to the indicated cell numbers in 100 µL volumes, delivered to the wells of a microplate, and incubated with AlamarBlue Cell Viability Reagent for 4 hr , fluorescence was measured at Ex/Em=545/590 nm.

To Order

Documents

Protocol (PDF):	A016
MSDS (PDF):	MSDS-A016
COA (PDF):	COA-A016

Reference:

Loss of PINK1 function promotes mitophagy through effects on oxidative stress and mitochondrial fission.

Dagda RK, Cherra SJ, Kulich SM, Tandon A, Park D, Chu CT,
J Biol Chem (2009) 284:13843-13855

The cross-linked biopolymer hyaluronic acid as an artificial vitreous substitute.

Schramm C, Spitzer MS, Henke-Fahle S, Steinmetz G, Januschowski K, Heiduschka P, Geis-Gerstorfer J, Biedermann T, Bartz-Schmidt KU, Szurman P
Invest Ophthalmol Vis Sci 2012 (53):613-621

MHC class I molecules act as tumor suppressor genes regulating the cell cycle gene expression, invasion and intrinsic tumorigenicity of melanoma cells.

Garrido C, Paco L, Romero I, Berruguilla E, Stefansky J, Collado A, Algarra I, Garrido F, Garcia-Lora AM
Carcinogenesis 2012 (33):687-693

Related Products

Buy	Cat.No.	Product Name	Ex/Em	Unit	Price
	A016-1	Cell-Quant™ AlamarBlue Cell Viability Reagent	405/450	500 assays	$55
	A016-2	Cell-Quant™ AlamarBlue Cell Viability Reagent	405/450	2500 assays	$180