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Calcium Indicators

Introduction

Intracellular Ca2+ plays a central role in cellular processes ranging from neuronal signaling and exocytosis to muscle contraction and bone formation. The fluorescent Ca2+ indicators have been widely used to study the role of Ca2+ in a specific cellular process.

ABP Biosciences has developed a number of calcium indicators to track Ca2+ concentrations with intense fluorescent signals and a range of wavelength options.
 

Calcium Indicators Selection Guide

Cat. No. Product Name Applic ation
C207, C208, C209 Fluo-2, AM Cell-permeant Ca2+ indicator, Kd: 230 nM, Ex/Em = 488/515 nm
C213, C214, C215 Fluo-3, AM Cell-permeant Ca2+ indicator, Kd: 390 nM, Ex/Em = 488/525 nm
C219, C220, C221 Fluo-4, AM Cell-permeant Ca2+ indicator, Kd: 345 nM, Ex/Em =  494/506 nm
C233, C234, C235 Rhod-2, AM Cell-permeant Ca2+ indicator, Kd: 570 nM, Ex/Em = 550/578 nm
C238 Coelenterazine Bioluminescent substrate for luciferases, used for calcium detection and the monitoring of reporter genes.


Figure 1. U2OS cells were seeded in a 96-well plate overnight, then incubated with Fluo-4 AM, followed by imaging with a fluorescence microscope using FITC channel.

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C207 Fluo-2, AM 488/515 1 mg $120 
C208 Fluo-2, AM 488/515 20×50 μg $150
C209 Fluo-2, AM, 1mg/ml in DMSO 488/515 1 ml $130
C213 Fluo-3, AM 488/525 1 mg $120
C214 Fluo-3, AM 488/525 20×50 μg $150
C215 Fluo-3, AM, 1mg/ml in DMSO 488/525 1 ml $130
C219 Fluo-4, AM 488/520 0.5 mg $100
C220 Fluo-4, AM 488/520 10×50 μg $130
C221 Fluo-4, AM, 1mg/ml in DMSO 488/520 0.5 ml $110
C233 Rhod-2, AM 550/578 1 mg $120
C234 Rhod-2, AM 550/578 20×50 μg $150
C235 Rhod-2, AM, 1mg/ml in DMSO 550/578 1 ml $130
C238 Coelenterazine   1 mg $150