Fluo-3, AM

Introduction

Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca²⁺. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-3, AM is supplied as 1 mg solid (C213), special packing 20×50 μg (C214), and 1 mM solution in DMSO (C215).

Specifications

Label:	Fluo-3
Ex/Em:	488/525
Detection Method:	Fluorescent
Solubility:	DMSO, DMF
Molecular Formula:	C₅₁H₅₀Cl₂N₂O₂₃
Molecular Weight:	1129.85
CAS Number:	121714-22-5
Storage Conditions:	-20 ℃, protect from light
Shipping Condition:	Room Temperature
Applications Calcium indicator

To order

Documents

Datasheet (PDF):	Fluo-3, AM
MSDS (PDF):	MSDS-C213, MSDS-C214, MSDS-C215

References:

Regulating T helper cell immunity through antigen responsiveness and calcium entry.

Bikah G, Pogue-Caley RR, McHeyzer-Williams LJ, McHeyzer-Williams MG
Nat Immunol (2000) 1:402-412

Calcium signaling of glial cells along mammalian axons.

Kriegler S, Chiu SY
J Neurosci (1993) 13:4229-4245

Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.

Lustig HS, von Brauchitsch KL, Chan J, Greenberg DA
J Neurochem (1992) 59:2193-2200

Related products

Buy	Cat.No.	Product name	Ex/Em(nm)	Size	Price
	C213	Fluo-3, AM	488/525	1 mg	$130
	C214	Fluo-3, AM	488/525	20×50 μg	$150
	C215	Fluo-3, AM, 1 mM in DMSO	488/525	1 ml	$140

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