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Fluo-3, AM

Introduction

Fluo-3, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-3 becomes fluorescent in the presence of free Ca2+. Fluo-3 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-3, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-3, AM is supplied as 1 mg solid (C213), special packing 20×50 μg (C214), and 1 mM solution in DMSO (C215).
 

Specifications

Label: Fluo-3
Ex/Em: 488/525
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C51H50Cl2N2O23
Molecular Weight: 1129.85
CAS Number: 121714-22-5
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Applications
Calcium indicator

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C213 Fluo-3, AM 488/525 1 mg $120
C214 Fluo-3, AM 488/525 20×50 μg $150
C215 Fluo-3, AM, 1 mM in DMSO 488/525 1 ml $130

Documents

Datasheet (PDF): Fluo-3, AM
MSDS (PDF): MSDS-C213, MSDS-C214, MSDS-C215

References:

Regulating T helper cell immunity through antigen responsiveness and calcium entry.
Bikah G, Pogue-Caley RR, McHeyzer-Williams LJ, McHeyzer-Williams MG
Nat Immunol (2000) 1:402-412
Calcium signaling of glial cells along mammalian axons.
Kriegler S, Chiu SY
J Neurosci (1993) 13:4229-4245

Ethanol and excitotoxicity in cultured cortical neurons: differential sensitivity of N-methyl-D-aspartate and sodium nitroprusside toxicity.
Lustig HS, von Brauchitsch KL, Chan J, Greenberg DA
J Neurochem (1992) 59:2193-2200

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