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Fluo-4, AM


Fluo-4, AM is a membrane permeant probe and can be passively loaded in cells by simple incubation. Once inside the cell, Fluo-4 becomes fluorescent in the presence of free Ca2+. Fluo-4 AM is widely used in flow cytometry, confocal laser scanning microscopy and fluorescence spectroscopy. Fluo-4, AM has proved particularly valuable for determining GPCR function via cell-based high-throughput screening.
Fluo-4, AM is supplied as 0.5 mg solid (C219), special packing 10×50 μg (C220), and 1 mg/ml solution in DMSO (C221).


Label: Fluo-4
Ex/Em: 494/506
Detection Method: Fluorescent
Solubility: DMSO, DMF
Molecular Formula: C51H50F2N2O23
Molecular Weight: 1096.95
CAS Number: 273221-67-3
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Calcium indicator

To order

Buy Cat.No. Product name Ex/Em(nm) Size Price
C219 Fluo-4, AM 496/506 0.5 mg $110
C220 Fluo-4, AM 496/506 10×50 μg $140
C221 Fluo-4, AM, 1 mg/ml in DMSO 496/506 0.5 ml $120


Datasheet (PDF): Fluo-4, AM


Targeted bulk-loading of fluorescent indicators for two-photon brain imaging in vivo.

Garaschuk O, Milos RI, Konnerth A
Nat Protoc (2006) 1:380-386

Local potassium signaling couples neuronal activity to vasodilation in the brain.

Filosa JA, Bonev AD, Straub SV, Meredith AL, Wilkerson MK, Aldrich RW, Nelson MT
Nat Neurosci (2006) 9:1397-1403

Negative regulation of Fc epsilonRI-mediated signaling and mast cell function by the adaptor protein LAX.

Zhu M, Rhee I, Liu Y, Zhang W
J Biol Chem (2006) 281:18408-18413

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