The interaction between biotin and avidin or streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the avidin-biotin interaction to be used for the development of robust and highly sensitive assays. The avidin-biotin detection system allows an almost unlimited number of primary detection reagents (i.e., antibodies, nucleic acid probes and ligands) to be easily captured, immobilized or detected with secondary detection reagents generated by modifying avidin or streptavidin.
Biotin Derivatives are employed in biotechnology as biotinylation reagents. ABP Biosciences offers many biotin reagents to facilitate biotinylation in the lab.
Biotin Derivative Selection Guide
Figure 1. Application of biotin derivatives
Cat. No.
Product Name
Application
Unit Size
C403
Biotin-11-dUTP, 1 mM in TE buffer
TUNEL, FISH
50 µL
C404
Biotin-11-UTP, 1 mM in TE buffer
TUNEL, FISH
50 µL
A031
Biotin-X Annexin V
Apoptosis
25 µg
C303
Biotin Alkyne
Click labeling
5 mg
C304
Biotin Azide
Click labeling
5 mg
C330
Biotin DBCO
Click labeling
1 mg
B003
Biotin, SE
Biotinylation reagent
100 mg
B004
Biotin-X, SE
Biotinylation reagent
100 mg
B005
Biotin-XX, SE
Biotinylation reagent
50 mg
B006
Biotin-PEG3, SE
Biotinylation reagent
25 mg
Buy
Cat.No.
Product name
Ex/Em (nm)
Size
Price
B003
Biotin, SE
–
100 mg
$88
B004
Biotin-X, SE
–
100 mg
$120
B005
Biotin-XX, SE
–
50 mg
$175
B006
Biotin-PEG3, SE
–
25 mg
$175
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