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Biotin Alkyne


Click chemistry describes a class of chemical reactions that use bio-orthogonal or biologically unique moieties to label and detect a molecule of interest in mild, aqueous conditions. The click reaction involves a copper-catalyzed triazole formation from an azide and an alkyne. The azide and alkyne moieties can be used interchangeably; either one can be used to tag the molecule of interest, while the other is used for subsequent detection.
The biotin alkyne is reactive with azide via a copper-catalyzed click reaction. Biotin can be subsequently detected with streptavidin, avidin or NeutrAvidin® biotin-binding protein.


  • Efficiency—the click reaction is complete in less than 1 hour;
  • Specificity—the reaction between the label and detection tag is selective and specific;
  • Stability—the reaction product contains an irreversible, covalent bond;
  • Biologically inert—the components of the reaction do not undergo any side reactions.

Figure 1. Click chemistry labeling


Label: Biotin
Detection Method:
Solubility: DMSO, DMF
Molecular Weight: 413.53
Product Size: 5 mg
Storage Conditions: -20 ℃, protect from light
Shipping Condition: Room Temperature

Click chemistry labeling


To order

Buy Cat.No. Product Name Ex/Em(nm) Unit Price
C303 Biotin Alkyne 5 mg $100


Datasheet (PDF): C303
MSDS (PDF): C303


Direct in-gel fluorescence detection and cellular imaging of O-GlcNAc-modified proteins.
Clark PM, Dweck JF, Mason DE, Hart CR, Buck SB, Peters EC, Agnew BJ, Hsieh-Wilson LC,
J Am Chem Soc (2008) 130:11576-11577

Robust fluorescent detection of protein fatty-acylation with chemical reporters.
Charron G, Zhang MM, Yount JS, Wilson J, Raghavan AS, Shamir E, Hang HC,
J Am Chem Soc (2009) 131:4967-4975

Regulation of calcium/calmodulin-dependent kinase IV by O-GlcNAc modification.
Dias WB, Cheung WD, Wang Z, Hart GW,
J Biol Chem (2009) 284:21327-21337

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