The interaction between biotin and avidin or streptavidin has been exploited for use in many protein and nucleic acid detection and purification methods. Because the biotin label is stable and small, it rarely interferes with the function of labeled molecules enabling the avidin-biotin interaction to be used for the development of robust and highly sensitive assays. The avidin-biotin detection system allows an almost unlimited number of primary detection reagents (i.e., antibodies, nucleic acid probes and ligands) to be easily captured, immobilized or detected with secondary detection reagents generated by modifying avidin or streptavidin.
 
	Biotin Derivatives are employed in biotechnology as biotinylation reagents. ABP Biosciences offers many biotin reagents to facilitate biotinylation in the lab.
 
	 
	Biotin Derivative Selection Guide
 
	 
					 
	 
	
	Figure 1. Application of biotin derivatives
 
				Cat. No.
			 
				Product Name
			 
				Application
			 
				Unit Size
			 
 
				C403
			 
				Biotin-11-dUTP, 1 mM in TE buffer
			 
				TUNEL, FISH
			 
				50 µL
			 
 
				C404
			 
				Biotin-11-UTP, 1 mM in TE buffer
			 
				TUNEL, FISH
			 
				50 µL
			 
 
				A031
			 
				Biotin-X Annexin V
			 
				Apoptosis
			 
				25 µg
			 
 
				C303
			 
				Biotin Alkyne
			 
				Click labeling
			 
				5 mg
			 
 
				C304
			 
				Biotin Azide
			 
				Click labeling
			 
				5 mg
			 
 
				C330
			 
				Biotin DBCO
			 
				Click labeling
			 
				1 mg
			 
 
				B003
			 
				Biotin, SE
			 
				Biotinylation reagent
			 
				100 mg
			 
 
				B004
			 
				Biotin-X, SE
			 
				Biotinylation reagent
			 
				100 mg
			 
 
				B005
			 
				Biotin-XX, SE
			 
				Biotinylation reagent
			 
				50 mg
			 
 
				B006
			 
				Biotin-PEG3, SE
			 
				Biotinylation reagent
			 
				25 mg
			 
 
				Buy
			 
				Cat.No.
			 
				Product name
			 
				Ex/Em (nm)
			 
				Size
			 
				Price
			 
 
				
			 
				B003
			 
				Biotin, SE
			 
				–
			 
				100 mg
			 
				$88
			 
 
				
			 
				B004
			 
				Biotin-X, SE
			 
				–
			 
				100 mg
			 
				$120
			 
 
				
			 
				B005
			 
				Biotin-XX, SE
			 
				–
			 
				50 mg
			 
				$175
			 
 
				
			 
				B006
			 
				Biotin-PEG3, SE
			 
				–
			 
				25 mg
			 
				$175
			 
 
 
 
				
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