Annexin V Binding Assays | ABP Biosciences

Introduction

The annexin V is a 35–36 kilodalton, Ca²⁺-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine (PS). In normal viable cells, phosphatidylserine is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, phosphatidylserine is translocated from the inner to the outer leaflet of the plasma membrane, exposing phosphatidylserine to the external cellular environment where it can be detected by annexin V conjugate (Figure 1).

Our fluorescent annexin V conjugates and kits provide quick and reliable detection methods for studying the externalization of phosphatidylserine in apoptotic cells. These conjugates and kits are very useful for flow cytometry or fluorescence microscopy to assess mixed populations of apoptotic and nonapoptotic cells.

Features:

Sensitivity—conjugated to Andy Fluor™ dyes for brighter signals
Flexibility—conjugates for many lasers and detection channels
Simplicity—assay procedure typically completed in less than 30 minutes

Figure 1. PS translocation in apoptotic cell.

Table 1. Selection Guide for Annexin V Binding Assay Kit

Cat. No.	Product Name	Probe for Apoptotic Cells (Ex/Em, nm)	Probe for Necrotic Cells (Ex/Em, nm)	Unit
A025	eGFP Annexin V and PI Apoptosis Kit	eGFP Annexin V (475/509)	PI (535/617)	100 assays
A026	FITC Annexin V and PI Apoptosis Kit	FITC Annexin V (490/519)	PI (535/617)	100 assays
A027	Andy Fluor™ 488 Annexin V and PI Apoptosis Kit	Andy Fluor™ 488 Annexin V (495/520)	PI (535/617)	100 assays
A028	Apoptotic, Necrotic, and Healthy Cells Detection Kit	Andy Fluor™ 488 Annexin V (495/520)	PI (535/617)	50 assays

Table 2. Selection Guide for Annexin V Reagent

Cat. No.	Product Name	Laser	Emission	Recommended filter	Unit
A029-1 A029-2	Annexin V, recombinant	–	–	–	0.5 mg 1.0 mg
A030	eGFP Annexin V	488 nm	509 nm	530/30 nm	100 assays
A031	Biotin-X Annexin V	–	–	–	100 assays
A032	Andy Fluor™ 350 Annexin V	UV	445 nm	450/40 nm	100 assays
A033	FITC Annexin V	488 nm	519 nm	530/30 nm	100 assays
A034	Andy Fluor™ 488 Annexin V	488 nm	520 nm	530/30 nm	100 assays
A035	Andy Fluor™ 555 Annexin V	532 nm	565 nm	575/26 nm	100 assays
A036	Andy Fluor™ 568 Annexin V	561 nm	603 nm	610/20 nm	100 assays
A037	Andy Fluor™ 594 Annexin V	590 nm	617 nm	630/20 nm	100 assays
A038	Andy Fluor™ 647 Annexin V	633 nm	665 nm	661/8 nm	100 assays
A039	Andy Fluor™ 680 Annexin V	635 nm	700 nm	700/20 nm	100 assays

To Order

Related Products

Buy	Cat. No.	Product Name	Ex/Em (nm)	Unit Size	Price
	A025	eGFP Annexin V and PI Apoptosis Kit	475/509	100 assays	$200
	A026	FITC Annexin V and PI Apoptosis Kit	490/519	100 assays	$200
	A027	Andy Fluor™ 488 Annexin V and PI Apoptosis Kit	495/520	100 assays	$200
	A028	Apoptotic, Necrotic, and Healthy Cells Detection Kit	–	50 assays	$165
	A029-1	Annexin V, recombinant	–	0.5 mg	$700
	A029-2	Annexin V, recombinant	–	1.0 mg	$1300
	A030	eGFP Annexin V, 100 assays	475/509	25 μg	$150
	A031	Biotin-X Annexin V, 100 assays	–	25 μg	$150
	A032	Andy Fluor™ 350 Annexin V, 100 assays	347/440	25 μg	$150
	A033	FITC Annexin V, 100 assays	490/519	25 μg	$150
	A034	Andy Fluor™ 488 Annexin V, 100 assays	495/520	25 μg	$150
	A035	Andy Fluor™ 555 Annexin V, 100 assays	553/565	25 μg	$150
	A036	Andy Fluor™ 568 Annexin V, 100 assays	578/602	25 μg	$150
	A037	Andy Fluor™ 594 Annexin V, 100 assays	590/615	25 μg	$150
	A038	Andy Fluor™ 647 Annexin V, 100 assays	650/665	25 μg	$150
	A039	Andy Fluor™ 680 Annexin V, 100 assays	680/700	25 μg	$150
	A041	5X Annexin V Binding Buffer	–	50 mL	$45