Our eGFP Annexin V and PI Apoptosis Kit is used to detect the externalization of phosphatidylserine in apoptotic cells. This kit provides a sensitive two-color assay that employs eGFP-annexin V and a ready-to-use solution of the red-fluorescent propidium iodide nucleic acid stain. Propidium iodide is impermeant to live cells and apoptotic cells but stains necrotic cells with red fluorescence, binding tightly to the nucleic acids in the cell. After staining a cell population with eGFP-annexin V and propidium iodide in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometer with the 488 nm spectral line of an argon-ion laser for excitation.
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Figure 1. Jurkat cells treated with 10 μM camptothecin for four hours (right panel) or untreated (as control, left panel). Cells were then treated with the reagents in the kit, followed by flow cytometric analysis.
| Protocol (PDF): | A025 |
| MSDS (PDF): |
Component A Component B Component C |
| COA (PDF): | A025 |
Cited Reference:
Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Sema Serter Kocoglu, Mücahit Seçme, Levent Elmas
Arch Biol Sci. 2022;74(3):227-234; https://doi.org/10.2298/ABS220219021S
Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Serter Koçolu S, Seçme M, Elmas L.
Arch Biol Sci 2022, 74(3):227-34. https://doi.org/10.2298/ABS220219021S
Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies
Zou L, Cheng G, Xu C, Liu H, Wang Y, Li N, Fan X, Zhu C, Xia W
Int J Nanomedicine. 2021, 16:1565-1573. doi: 10.2147/IJN.S292319
Activation of Aquaporin 5 by carcinogenic Helicobacter pylori infection promotes epithelial-mesenchymal transition via the MEK/ERK pathway
Li, N., Xu, X., Yang, H., Wang, H., Ouyang, Y., Zhou, Y., … Hong, J.
Helicobacter. 2021,00:e12842. https://doi.org/10.1111/hel.12842
Fresh and cultured mouse islets differ in their response to nutrient stimulation
Morsi M, Schulze T, Früh E, Brüning D, Panten U, Rustenbeck I.
Endocr Connect. 2020, 9(8):769-782. doi: 10.1530/EC-20-0289
Naringin induces endoplasmic reticulum stress-mediated apoptosis, inhibits catenin pathway and arrests cell cycle in cervical cancer cells
Ruyin Lin, Xinxin Hu, Shaorong Chen, Qiyang Shi and Huiqing Chen
Acta Biochimica Polonica, 2020, 67, 181-188. https://doi.org/10.18388/abp.2020_5182
Pharmacological inhibition of thioredoxin reductase increases insulin secretion and diminishes beta cell viability
Brüning, D., Hatlapatka, K., Lier-Glaubitz, V. et al.
Naunyn-Schmiedeberg's Arch Pharmacol 394, 1133–1142 (2021). https://doi.org/10.1007/s00210-020-02046-2
MicroRNA function can be reversed by altering target gene expression levels
Alexander A. Svoronos, Stuart G. Campbell, Donald M. Engelman
iScience, 2021, 24, 10, 103208, https://doi.org/10.1016/j.isci.2021.103208
Monensinin Glioblastoma Multiformede Kaspaz-10 Aracılı Apoptoz Üzerine Etkileri
Sema SERTER KOÇOLU, Mücahit SEÇME
Uluda Üniversitesi Tıp Fakültesi Dergisi, 47 (3) 393-397, 2021. DOI: https://doi.org/10.32708/uutfd.999511
[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
SSRN: http://dx.doi.org/10.2139/ssrn.4102564
- FITC Annexin V and PI Apoptosis Kit
- Andy Fluor™ 488 Annexin V and PI Apoptosis Kit
- Apoptotic, Necrotic, and Healthy Cells Detection Kit
- Annexin V, recombinant
- eGFP Annexin V
- Biotin-X Annexin V
- Andy Fluor™ 350 Annexin V
- FITC Annexin V
- Andy Fluor™ 488 Annexin V
- Andy Fluor™ 555 Annexin V
- Andy Fluor™ 568 Annexin V
- Andy Fluor™ 594 Annexin V
- Andy Fluor™ 647 Annexin V
- Andy Fluor™ 680 Annexin V
