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Apoptotic, Necrotic, and Healthy Cells Detection Kit


Our Apoptotic, Necrotic, and Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent:  Andy Fluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence.


  • Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay
  • Fast: A single 30 min staining step
  • Sensitive: Brighter fluorescence signal


Platform: Fluorescence Cytometer, Fluorescence Microscopy
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm
Product Size: 50 assays
Storage Conditions: 4 ℃, protect from light
Shipping Condition: Ice pack

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A028 Apoptotic, Necrotic, and Healthy Cells Detection Kit 495/520 50 assays $150


Protocol (PDF): A028
MSDS (PDF):  Component A
Component B
Component C
Component D
COA (PDF):  A028


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J Am Chem Soc (2007) 129:2187-2193

Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
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J Immunol Methods (1999) 229:81-95