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Apoptotic, Necrotic, and Healthy Cells Detection Kit


Our Apoptotic, Necrotic, and Healthy Cells Detection Kit provides a convenient assay to detect apoptotic, necrotic, and total cells within the same cell population by flow cytometry or fluorescence microscopy. This kit contains three-color reagent:  Andy Fluor™ 488 annexin V used to stain apoptotic cells by binding to phosphatidylserine (PS) exposed on the cell surface; propidium iodide (PI) used to stain necrotic cells with comprised membrane integrity; and Hoechst 33324 used to stain all cell nuclei. After staining a cell population, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and all cells show blue fluorescence.


  • Simple: Simultaneous detection of apoptotic, necrotic, and total cells in a single assay
  • Fast: A single 30 min staining step
  • Sensitive: Brighter fluorescence signal


Platform: Fluorescence Cytometer, Fluorescence Microscopy
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; Andy Fluor™ 488: 495/520 nm; Hoechst 33324: 350/460 nm
Product Size: 50 assays
Storage Conditions: 4 ℃, protect from light
Shipping Condition: Ice pack

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A028 Apoptotic, Necrotic, and Healthy Cells Detection Kit 495/520 50 assays $165



Protocol (PDF): A028
MSDS (PDF):  Component A
Component B
Component C
Component D
COA (PDF):  A028

​Cited Reference:

Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Sema Serter Kocoglu, Mücahit Seçme, Levent Elmas
Arch Biol Sci. 2022;74(3):227-234; https://doi.org/10.2298/ABS220219021S

Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
Serter Koçolu S, Seçme M, Elmas L.
Arch Biol Sci 2022, 74(3):227-34. https://doi.org/10.2298/ABS220219021S

Copper Nanoparticles Induce Oxidative Stress via the Heme Oxygenase 1 Signaling Pathway in vitro Studies
Zou L, Cheng G, Xu C, Liu H, Wang Y, Li N, Fan X, Zhu C, Xia W
Int J Nanomedicine. 2021, 16:1565-1573. doi: 10.2147/IJN.S292319

Activation of Aquaporin 5 by carcinogenic Helicobacter pylori infection promotes epithelial-mesenchymal transition via the MEK/ERK pathway
Li, N., Xu, X., Yang, H., Wang, H., Ouyang, Y., Zhou, Y., … Hong, J.
Helicobacter. 2021,00:e12842. https://doi.org/10.1111/hel.12842

Fresh and cultured mouse islets differ in their response to nutrient stimulation
Morsi M, Schulze T, Früh E, Brüning D, Panten U, Rustenbeck I.
Endocr Connect. 2020, 9(8):769-782. doi: 10.1530/EC-20-0289

Naringin  induces  endoplasmic  reticulum  stress-mediated  apoptosis,  inhibits  catenin  pathway  and  arrests  cell  cycle  in  cervical  cancer  cells
Ruyin Lin, Xinxin Hu, Shaorong Chen, Qiyang Shi and Huiqing Chen
Acta Biochimica Polonica, 2020, 67, 181-188. https://doi.org/10.18388/abp.2020_5182

Pharmacological inhibition of thioredoxin reductase increases insulin secretion and diminishes beta cell viability
Brüning, D., Hatlapatka, K., Lier-Glaubitz, V. et al.
Naunyn-Schmiedeberg's Arch Pharmacol 394, 1133–1142 (2021). https://doi.org/10.1007/s00210-020-02046-2

MicroRNA function can be reversed by altering target gene expression levels
Alexander A. Svoronos, Stuart G. Campbell, Donald M. Engelman
iScience, 2021, 24, 10, 103208, https://doi.org/10.1016/j.isci.2021.103208

Monensinin Glioblastoma Multiformede Kaspaz-10 Aracılı Apoptoz Üzerine Etkileri
Uluda Üniversitesi Tıp Fakültesi Dergisi, 47 (3) 393-397, 2021. DOI: https://doi.org/10.32708/uutfd.999511

[11]-Chaetoglobosins with Cytotoxic Activities from Pseudeurotium Bakeri
Ruan, Hanli and Duan, Fangfang and Gao, Ying and Peng, Xiaogang and Meng, Xianggao and Chang, Jinling and Gan, Yutian and Ouyang, Qianxi
SSRN: http://dx.doi.org/10.2139/ssrn.4102564