A distinctive feature of the early stages of apoptosis is the disruption of active mitochondria, including changes in membrane potential and alterations to redox potential. JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide) is a novel cationic carbocyanine dye. In healthy cells, JC-1 selectively accumulates in mitochondria and forms J-aggregates that exhibit red fluorescence.
In apoptotic cells with reduced mitochondria membrane potential, JC-1 exists as a monomer resulting in green fluorescence. These characteristics make JC-1 a sensitive marker for mitochondrial membrane potential.
- Simple workflow: add, incubate, and read format, no wash needed.
- Flexibility: suitable for flow cytometry, fluorescence microscope, and plate reader.
- Fast: results in as little as 30 minutes.
ABP Biosciences offers a selection of mitichondrial probes:
Figure 1. Detection of cell apoptosis with JC-1 Mitochondrial Membrane Potential Detection Kit. Jurkat cells treated with 10 μM camptothecin for 4 hr (right panel) or untreated (left panel). Cells were stained with JC-1 reagent, then analyzed by flow cytometry.