• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on CDCFDA

Introduction To Order Documents Related Products Introduction 5(6)-CDCFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-CDCFDA yields the fluorescent […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on CFDA, SE

Introduction To Order Documents Related Products Introduction CFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. CFDA, SE passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, this probe […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on CFDA

Introduction To Order Documents Related Products Introduction 5(6)-CFDA is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, the 5(6)-carboxyfluorescein diacetate yields 5(6)-carboxyfluorescein. […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on BCECF, AM

Introduction To Order Documents Related Products Introduction BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on BCECF

Introduction To Order Documents Related Products Introduction BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Calcein, AM

Introduction To Order Documents Related Products Introduction Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells the nonfluorescent calcein AM is converted to a green-fluorescent calcein, after acetoxymethyl ester hydrolysis by intracellular esterases. This dye is provided as 1 mg solid (C025) and […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Calcein blue, AM

Introduction To Order Documents Related Products Introduction Calcein Blue, AM is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Calcein blue AM is only weakly fluorescent (excitation/emission maxima ~322/435 nm). Upon cleavage of the […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on Pluronic F-127®

Introduction To Order Documents Related Products Introduction Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media. Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of ion indicators as well as the […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiR iodide

Introduction To Order Documents Related Products Introduction DiR is a near IR fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, […]

• YLQ
• Cell Structure Probes, Product
• Oct, 16, 2014
• Comments Off on DiD perchlorate

Introduction To Order Documents Related Products Introduction DiD is a far-red fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes.  It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the […]