Introduction To Order Documents Related Products Introduction The Quick T4 DNA Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 15 minutes at room temperature. Applications Routine subcloning. Recircularization of linear DNA. Library construction. Linker ligation. Quick Ligation Protocol Combine 50 ng of vector with a 3-fold molar excess of insert. […]
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T4 DNA Ligase
Introduction To Order Documents Related Products Introduction T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini. The T4 DNA Ligase requires ATP as […]
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PolyFectRNA™ Transfection Reagent
Introduction To Order Documents Related Products Introduction PolyFectRNA™ Transfection Reagent is an innovative cationic nanotechnology developed specifically for the delivery of siRNA and miRNA into a wide range of eukaryotic cells. PolyFectRNA™ Transfection Reagent provides the highest transfection efficiencies on the widest variety of cell types for siRNA-mediated gene knockdown experiments. The reagent is compatible […]
Continue Reading PolyFectVIR™ Transfection Reagent
Introduction To Order Documents Related Products Introduction PolyFectVIR™ Transfection Reagent is an innovative cationic nanotechnology developed for the gene and cell therapy field to improve virus production. This ready-to-use chemically defined reagent is guaranteed free of components of animal origin. PolyFectVIR™ Transfection Reagent is dedicated for medium to large scale bioproduction of virus through an […]
Continue Reading Benzonase Nuclease
Introduction To Order Documents Related Products Introduction Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. It is effective over a wide range of conditions and possesses an exceptionally high specific activity. The enzyme […]
Continue Reading T4 β-Glucosyltransferase
Introduction To Order Documents Related Products Introduction T4 β-glucosyltransferase (T4 BGT) is a DNA-modifying enzyme encoded by bacteriophage T4 that catalyzes the transfer of glucose (Glc) from uridine diphosphoglucose (UDP-Glc) to 5-hydroxymethylcytosine (5-hmC) residues in double-stranded DNA, resulting in the formation of β-glucosyl-5- hydroxymethylcytosine. The enzyme is available in 500 and 2,500 unit sizes at […]
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Modified Nucleoside-5′-Triphosphates
Introduction To Order Related Products Introduction Base modified NTPs have been widely used for synthesis of modified mRNA to improve stability and reduce immunogenicity. ABP Biosciences offers a list of key base modified NTPs for mRNA synthesis. Cat. No. Product Name Unit Size LP009-1 N1-Methylpseudo-UTP 10 µL LP009-2 N1-Methylpseudo-UTP 50 µL LP009-3 N1-Methylpseudo-UTP 100 µL […]
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5-Methyl-CTP
Introduction To order Documents Related Products Introduction 5-Methyl-CTP (5-Methylcytidine-5'-Triphosphate) is a modified nucleoside triphosphate employed to impart desirable characteristics such as increased nuclease stability, increased translation or reduced interaction of innate immune receptors with in vitro transcribed RNA. 5-Methyl-CTP, as well as Pseudo-UTP and N1-Methylpseudo-UTP, have been shown to reduce innate immune stimulation in culture […]
Continue Reading Pseudo-UTP
Introduction To order Documents Related Products Introduction Pseudo-UTP (Pseudouridine-5'-Triphosphate) is one of the most commonly used modified nucleoside-5’-O-triphosphates. Pseudo-UTP is a building block for the polymerase-mediated synthesis of RNA molecules. RNA molecules, especially messenger RNAs (mRNAs), have widespread applications in therapeutic and diagnostic areas, and have demonstrated their superiority over plasmid DNA techniques in both […]
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N1-Methylpseudo-UTP
Introduction To order Documents Related Products Introduction N1-Methylpseudo-UTP (N1-Methyl-Pseudouridine-5'-Triphosphate) is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA. It was reported that N1-methylpseudouridine (m1Ψ) modification alone or in combination with 5-methylcytidine (m5C) exhibited superiority over the current state-of-the-art pseudouridine […]
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