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N1-Methylpseudo-UTP (N1-Methyl-Pseudouridine-5'-Triphosphate) is a modified NTP for incorporation into messenger RNAs (mRNA) using T7 RNA Polymerase. Incorporation of N1-methylpseudouridine can reduce the immunogenicity of the resulting mRNA.

It was reported that N1-methylpseudouridine (m1Ψ) modification alone or in combination with 5-methylcytidine (m5C) exhibited superiority over the current state-of-the-art pseudouridine (Ψ) or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) and ~13-fold (when comparing single modified mRNAs) higher reporter gene expression in cells and mice, respectively. Moreover, compared with (m5C/)Ψ-modified mRNAs, (m5C/)m1Ψ-modified mRNAs showed reduced intracellular innate immunogenicity and resulted in improved cellular viability after in vitro transfection. Thus, N1-methylpseudo-UTP might serve as a useful ingredient for synthesizing drugable mRNAs with better performance. 


1. Andries O, Mc Cafferty S, De Smedt SC, et al. N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice. Journal of Controlled Release, 2015, 217: 337-344.

For research use only.


Technical Information

Formal Name: N1-Methyl-Pseudouridine-5'-Triphosphate
CAS Number: 1428903-59-6 (free acid)
Molecular Formula: C10H17N2O15P3 (free acid)
Molecular Weight: 497.98 (free acid)
Purity: >95%
Extinction Coefficient: 8,880 Lmol-1cm-1 at 271 nm
Salt Form: Na+
Concentration: 100 mM in H2O
Storage Condition: -20 ℃
Shipping Condition: Ice packs

To order

Buy Cat.No. Product name Size Price
LP009-1 N1-Methylpseudo-UTP 10 µL $90
LP009-2 N1-Methylpseudo-UTP 50 µL $400
LP009-3 N1-Methylpseudo-UTP 100 µL $740


Product Information (PDF): LP009

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