PolyFect™ Transfection Reagent

Introduction

PolyFect™ Transfection Reagent is a biodegradable polymer based transfection reagent that forms a complex with DNA, and transports the complex into a variety of adherent and suspension cell lines. A remarkable feature of the reagent is the rapid and complete degradation of polymer after transfection complex endocytosis, leading to much less cytotoxicity.

Features

Superior transfection efficiency for a broad range of cell lines
Does not require removal of serum or culture medium
Does not require washing or changing of medium after transfection
Low cytotoxicity

Figure 1. Comparison of PolyFect™ and Lipofectamine® 2000 transfection efficiencies. Expi293F, CHO, A549, NIH-3T3, COS-7, HepG2 cells were transfected with a GFP-expressing plasmid using either PolyFect™ transfection reagent (yellow), or Lipofectamine® 2000 (blue). Cells were evaluated for transfection efficiencies after 48 hours post-transfection.

Figure 2. The transfection efficiencies of PolyFect™ in Expi293F, and CHO suspension cells are over 95%, ideal for protein expression.

To Order

Documents

Protocol (PDF):	FP330	FP331
MSDS (PDF):	MSDS-FP330	MSDS-FP331

Cited Reference:

Bioinformatics analysis of LINC01554 and its co‑expressed genes in hepatocellular carcinoma
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Sesterterpene MHO7 suppresses breast cancer cells as a novel estrogen receptor degrader
Yue Zhao, Chenxi Zhao, Jin Lu, Jun Wu, Changhao Li, Zhiye Hu, Wei Tian, Liang Yang, Jin Xiang, Haibin Zhou, Zixin Deng, Jian Huang, Kui Hong
Pharmacological Research, 2019, 146, 104294, https://doi.org/10.1016/j.phrs.2019.104294

Generation of C-to-G transversion in mouse embryos via CG editors
Cao, T., Liu, S., Qiu, Y. et al.
Transgenic Res 31, 445–455 (2022). https://doi.org/10.1007/s11248-022-00313-x

RIPK3 Promotes JEV Replication in Neurons via Downregulation of IFI44L
Bian P, Ye C, Zheng X, Luo C, Yang J, Li M, Wang Y, Yang J, Zhou Y, Zhang F, Lian J, Zhang Y, Jia Z and Lei Y
Front. Microbiol. 2020, 11:368. doi: 10.3389/fmicb.2020.00368

Stanniocalcin 2 Is Upregulated by Calcium-Sensing Receptor and Protects Human Vascular Smooth Muscle Cells from High-Phosphate-Induced Apoptosis
Liu F, Zhang A, Huang H, Liu X, Diao Z, Deng D, Liu W
Cardiorenal Med 2022;12:214-228. doi: 10.1159/000527441

STC2: A Novel and Regulable Protector of Vascular Smooth Muscle Cells
Zhang, Ai-hua and Liu, Fen-fen and Huang, Hong-dong and Liu, Xu and Diao, Zong-li and Deng, Dai and Liu, Wen-hu
http://dx.doi.org/10.2139/ssrn.4010831

Axl-/- neurons promote JEV infection by dampening the innate immunity
Jiali Yang, Mengyuan Li, Mingcheng Yuan, Peiyu Bian, Yangchao Dong, Haijun Zhang, Chuanyu Luo, Zhifeng Xue, Yuan Wang, Fanglin Zhang, Lixin Shen, Yingfeng Lei
Virus Research, 2022, 307, 198605, https://doi.org/10.1016/j.virusres.2021.198605

Endothelial and systemic upregulation of miR-34a-5p fine-tunes senescence in progeria.
Manakanatas C, Ghadge SK, Agic A, Sarigol F, Fichtinger P, Fischer I, Foisner R, Osmanagic-Myers S.
Aging (Albany NY). 2022 Jan 12;14(1):195-224. doi: 10.18632/aging.203820

A novel homozygous RIPK4 variant in a family with severe Bartsocas-Papas syndrome
Dinçer, T., Gümüş, E., Toraman, B., Er, Yildiz, G., Yüksel, Z., & Kalay, E.
Am J Med Genet. 2021;185A:1691–1699. doi:10.1002/ajmg.a.62154

Comprehensive Pan-Cancer Analyses of Immunogenic Cell Death as a Biomarker in Predicting Prognosis and Therapeutic Response.
Wang, Y.; Huang, Y.; Yang, M.; Yu, Y.; Chen, X.; Ma, L.; Xiao, L.; Liu, C.; Liu, B.; Yuan, X.
Cancers 2022, 14, 5952. https://doi.org/10.3390/cancers14235952

Hydroxychloroquine impairs mTORC1-dependent cholesterol biosynthesis and sensitizes retinal pigment epithelial cells to UV-induced cell death
I Wicks, ML Kim, D De Souza, V Narayana, N Nguyen
Research Square, 2022, https://doi.org/10.21203/rs.3.rs-2299218/v1

PTPRH Alleviates Airway Obstruction and Th2 Inflammation in Asthma as a Protective Factor
Feng-jia Chen, Li-juan Du, Zhimin Zeng, Xin-yan Huang, Chang-yi Xu, Wei-ping Tan, Can-mao Xie, Yu-xia Liang & Yu-biao Guo
Journal of Asthma and Allergy, 2022, 15: 133-144. DOI: 10.2147/JAA.S340059

Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells
Xian-Zhang Wang, Le Wen, Yue-Peng Zhou, Sheng-Nan Huang, Bo Yang, Shuang Cheng, Wen-Bo Zeng, Meng-Jie Mei, Jin-Yan Sun, Xuan Jiang, Han Cheng, Min-Hua Luo
PLoS Pathog 2023, 19(2): e1011166. https://doi.org/10.1371/journal.ppat.1011166

Comprehensive Pan-Cancer Analyses of Immunogenic Cell Death as a Biomarker in Predicting Prognosis and Therapeutic Response.
Wang, Y.; Huang, Y.; Yang, M.; Yu, Y.; Chen, X.; Ma, L.; Xiao, L.; Liu, C.; Liu, B.; Yuan, X.
Cancers 2022, 14, 5952. https://doi.org/10.3390/cancers14235952

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Buy	Cat.No.	Product Name	Unit	Price
	FP330	PolyFect™ Transfection Reagent	1 mL	$175
	FP331	PolyFect™ Transfection Reagent	3 mL	$400