iScript™ IV TaqProbe One-Step RT-qPCR Kit is a complete qPCR system containing all necessary reagents for both reverse transcription and TaqMan Probe based qPCR amplification to occur in a single reaction qPCR reaction tube. RT-qPCR Enzyme Mix (10X) contains iScript™ IV Reverse Transcriptase (RT), Hot-Start Taq DNA Polymerase and RNase Inhibitor for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-qPCR Master Mix (5X) contains stabilizers and enhancers that optimize the two reactions in a real-time “single step”. This One-Step RT-qPCR Kit offers the end-users an efficient and reliable alternative to conventional “two-step” sequential RT-qPCR. Gene-specific primers must be used along with this kit.
Applications
- Gene-expression analysis.
- Transcription analysis.
- Gene cloning.
- Virus detection and quantification.
Related Products
- iScript™ IV Reverse Transcriptase
- iScript™ IV First-Strand cDNA Synthesis Kit
- iScript™ IV First-Strand cDNA Synthesis Kit with dsDNase
- iScript™ All-in-One RT Master Mix (5X)
- iScript™ IV One-Step RT-PCR Kit
- iScript™ IV SYBR Green One-Step RT-qPCR Kit
- iScript™ IV TaqProbe One-Step RT-qPCR Kit
