iScript™ IV One-Step RT-PCR Kit

iScript™ IV One-Step RT-PCR Kit contains all necessary reagents for both reverse transcription and PCR amplification to occur in a single reaction tube. RT-PCR Enzyme Mix (10X) contains iScript™ IV Reverse Transcriptase (RT), Hot-Start Taq DNA Polymerase and RNase Inhibitor for highly sensitive and specific RT-PCR using any RNA template. Our proprietary RT-PCR Reaction Mix (5X) contains stabilizers and enhancers that optimize the two reactions in a “single step”. Together with a specially formulated RT-PCR Reaction Mix, this One-Step RT-PCR Kit offers the end-users an efficient and reliable alternative to conventional “two-step” sequential RT-PCR.

Applications

• Gene-expression analysis.
• Transcription analysis.
• Gene cloning.
• Virus detection and quantification.