CDCFDA, SE is a fixable-cell-permeant, fluorescein-based tracer for very long-term cell labeling. CDCFDA, SE passively diffuse into cells and it is nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the highly fluorescent, amine-reactive fluorophore. Upon reaction with amine-containing residues of intracellular proteins, this probe is well retained in cells.
|Excitation/Emission:||504/529 nm (after cleavage)|
|Shipping Condition:||Ice pack|
|Storage Conditions:||-20ºC, protect from light|
Characterization of Vta1p, a class E Vps protein in Saccharomyces cerevisiae.
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J Biol Chem (2004) 279:10982-10990
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