BCECF is an ideal dual-excitation ratiometric pH indicator for measuring changes ± 1 pH unit around pH 7 with a pKa of ~6.98. pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm.
| Specifications: |
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| Excitation/Emission: | 490/535 | |
| Shipping Condition: | Ambient | |
| Storage Conditions: | 2-8ºC, protect from light | |
| Molecular Formula: | C27H20O11 | |
| Molecular Weight: | 520.45 | |
| CAS Number: | 85138-49-4 | |
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| Protocol (PDF): | C027 |
| MSDS (PDF): | MSDS-C027 |
| MSDS (PDF): | C027 |
Reference:
Fluorescent indicators for intracellular pH.
Han J, Burgess K,
Chem Rev (2010) 110:2709-2728
Wavelength Considerations in Confocal Microscopy of Botanical Specimens.
Fricker MD, White NS
J Microsc (1992) 166:29-29
Calibration Methods and Avoidance of Errors in Measurement of Intracellular pH (pHcyt) Using the Indicator Bis (Carboxyethyl)-5(6)-Carboxyfluorescein (BCECF) in Human Platelets.
Valant PA, Haynes DH
J Fluorescence (1992) 2:191-191
