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BCECF, AM

Introduction

BCECF, AM is an ideal cell-permeant, dual-excitation ratiometric pH indicator for measuring changes in the cytosolic pH of most cells. Intracellular pH measurements with BCECF are made by determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at ~490 nm versus the emission intensity when excited at its isobestic point of ~440 nm. This dye is provided as 1 mg solid (C028) and 1 mg/mL solution in DMSO (C029).

Specifications:     
Excitation/Emission: 490/535 (after cleavage)
Shipping Condition: Ice pack
Storage Conditions: -20ºC, protect from light
Molecular Formula: N/A
Molecular Weight: N/A
CAS Number: 117464-70-7  

 

To Order

Buy Cat.No. Product name Ex/Em(nm) Size Price

C028
C029
BCECF, AM 490/535 1 mg
500 µl
$140
$80

 
 
     

Documents

Protocol (PDF): C028  C029
MSDS (PDF):  MSDS-C028  MSDS-C029
COA (PDF):  C028 C029

Reference:

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Stage-specific expression of mucosal addressin cell adhesion molecule-1 during embryogenesis in rats.
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J Immunol (2000) 164:2463-2471