Annexin V, recombinant

Annexin V is a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

Our annexin V is provided as 0.5 mg (A029-1) or 1.0 mg (A029-2) size in lyophilized form.

Reconstitution:  We recommend a quick spin followed by reconstitution in 1 ml distilled water (incubate at 30°C for 5 minutes). Reconstituted solution contains 20 mM phosphate buffer (pH 7.0).
 

Specifications Source:                         E. coli Molecular Weight:      35.8 kDa Form: Lyophilized Purity:     >90%   Applications Cell apoptosis analysis.