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Annexin V, recombinant

Introduction

Annexin V is a family of calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In healthy cells, PS is predominantly located along the cytosolic side of the plasma membrane. Upon initiation of apoptosis, PS loses its asymmetric distribution in the phospholipid bilayer and translocates to the extracellular membrane, which is detectable with fluorescently labeled Annexin V. In early stages of apoptosis, the plasma membrane excludes viability dyes such as propidium iodide (PI) and 7-AAD, therefore cells which display only Annexin V staining (PI/7-AAD negative) are in early stages of apoptosis. During late-stage apoptosis, loss of cell membrane integrity allows Annexin V binding to cytosolic PS, as well as cell uptake of PI and 7-AAD. Annexin V staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry.

Our annexin V is provided as 0.5 mg (A029-1) or 1.0 mg (A029-2) size in lyophilized form.

Reconstitution:  We recommend a quick spin followed by reconstitution in 1 ml distilled water (incubate at 30°C for 5 minutes). Reconstituted solution contains 20 mM phosphate buffer (pH 7.0).
 

Specifications

Source:                         E. coli
Molecular Weight:      35.8 kDa
Form: Lyophilized
Purity:     >90%

 

Applications

Cell apoptosis analysis.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price

A029-1
A029-2
Annexin V, recombinant 0.5 mg
1.0 mg
$650
$1200

Documents

Protocol (PDF): A029
MSDS (PDF):  MSDS-A029

Reference

Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
Logue SE, Elgendy M, Martin SJ,
Nat Protoc (2009) 4:1383-1395