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Andy Fluor™ 488 Annexin V

Introduction

Andy Fluor™ 488 Annexin V is Annexin V conjugated with our green fluorescent dye Andy Fluor™ 488. Annexin V is a calcium-dependent phospholipid-binding proteins, which bind to phosphatidylserine (PS) to identify apoptotic cells. In normal viable cells, PS is located on the cytoplasmic surface of the cell membrane. In apoptotic cells, however, PS is translocated from the inner to the outer leaflet of the plasma membrane, making itself available for Annexin V binding.

Andy Fluor™ 488 is a superior alternative to the traditional green fluorescent dye FITC (or fluorescein) owing to its exceptional brightness and photostability. Andy Fluor™ 488 has brightness and photostability comparable to Alexa Fluor® 488.

Our Andy Fluor™ 488 Annexin V is provided as lyophilized power, which is enough for 100 assays.

Reconstitution:  We recommend a quick spin followed by reconstitution in 500 μl distilled water, and store at 4°C.

   

To order

Buy Catalog # Product Name Ex/Em (nm) Unit Size List Price
A034 Andy Fluor™ 488 Annexin V, 100 assays 495/520 25  μg $140

Documents

Protocol (PDF): A034
MSDS (PDF):  MSDS-A034
COA (PDF):  COA-A034

Reference

Expression, purification and use of recombinant annexin V for the detection of apoptotic cells.
Logue SE, Elgendy M, Martin SJ,
Nat Protoc (2009) 4:1383-1395

Flow cytometry-based apoptosis detection.
Wlodkowic D, Skommer J, Darzynkiewicz Z,
Methods Mol Biol (2009) 559:19-32

Fluorescent biomembrane probe for ratiometric detection of apoptosis.
Shynkar VV, Klymchenko AS, Kunzelmann C, Duportail G, Muller CD, Demchenko AP, Freyssinet JM, Mely Y,
J Am Chem Soc (2007) 129:2187-2193

Automatic image analysis for quantification of apoptosis in animal cell culture by annexin-V affinity assay.
Pläsier B, Lloyd DR, Paul GC, Thomas CR, Al-Rubeai M
J Immunol Methods (1999) 229:81-95