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eGFP Annexin V and PI Apoptosis Kit

Introduction

Our eGFP Annexin V and PI Apoptosis Kit is used to detect the externalization of phosphatidylserine in apoptotic cells. This kit provides a sensitive two-color assay that employs eGFP-annexin V and a ready-to-use solution of the red-fluorescent propidium iodide nucleic acid stain.  Propidium iodide is impermeant to live cells and apoptotic cells but stains necrotic cells with red fluorescence, binding tightly to the nucleic acids  in the cell. After staining a cell population with eGFP-annexin V and propidium iodide in the provided binding buffer, apoptotic cells show green fluorescence, dead cells show red and green fluorescence, and live cells show little or no fluorescence. These populations can easily be distinguished using a flow cytometer with the 488 nm spectral line of an argon-ion laser for excitation.
 

Specifications

Platform: Fluorescence Cytometer
Detection Method: Fluorescent
Ex/Em: PI: 535/617 nm; eGFP: 475/509 nm
Product Size: 100 assays
Storage Conditions: 4 ℃, protect from light
Shipping Condition: Ice pack

 

Applications

Cell apoptosis analysis.

 

Figure 1. Jurkat cells treated with 10 μM camptothecin for four hours (right panel) or untreated (as control, left panel). Cells were then treated with the reagents in the kit, followed by flow cytometric analysis.

To Order

Buy Cat.No. Product Name Ex/Em Unit Price
A025 eGFP Annexin V and PI Apoptosis Kit 475/509 100 assays $180

Documents

Protocol (PDF): A025
MSDS (PDF):  A025
COA (PDF):  A025

Reference:

Nuclear relocation of the nephrin and CD2AP-binding protein dendrin promotes apoptosis of podocytes.
Asanuma K, Campbell KN, Kim K, Faul C, Mundel P
Proc Natl Acad Sci U S A (2007) 104:10134-10139

Synthesis and identification of small molecules that potently induce apoptosis in melanoma cells through G1 cell cycle arrest.
Dothager RS, Putt KS, Allen BJ, Leslie BJ, Nesterenko V, Hergenrother PJ,
J Am Chem Soc (2005) 127:8686-8696

TRPV1 receptors mediate particulate matter-induced apoptosis.
Agopyan N, Head J, Yu S, Simon SA
Am J Physiol Lung Cell Mol Physiol (2004) 286:L563-L572