ExoFast™ Exosome Isolation Reagent from other body fluids enables fast and efficient enrichment of intact exosomes from cerebrospinal fluid (CSF), ascitic fluid, amniotic fluid, milk, and saliva with a protocol that is scalable depending on your sample size.
Advantages:
- Maximize recovery of intact exosomes for any type of downstream application
- Easily isolate exosomes using our simple and reliable protocol
- Avoid time-consuming ultra-centrifugation
- Maximize flexibility—can be scaled up or down depending on the sample size
By tying up water molecules, the reagent forces less-soluble components such as vesicles out of solution, allowing them to be collected by a short, low-speed centrifugation. The reagent is added to the body fluid, and the solution is incubated at 2–8°C or room temperature (temperature and length of incubation depend on specific body fluid). The precipitated exosomes are recovered by standard centrifugation at 10,000 x g. The pellet is then resuspended in PBS or similar buffer, and the exosomes are ready for downstream analysis or further purification by affinity methods.
| Protocol (PDF): | D034 |
| MSDS (PDF): | MSDS-D034 |
Cited Reference:
Exosome Derived from ADSCs Attenuates Ultraviolet B-mediated Photoaging in Human Dermal Fibroblasts
Gao, W., Wang, X., Si, Y., Pang, J., Liu, H., Li, S., … Wang, Y.
Photochemistry and Photobiology. 2021, 97, 795-804. doi:10.1111/php.13370
miR-1246-overexpressing exosomes suppress UVB-induced photoaging via regulation of TGF- β/Smad and attenuation of MAPK/AP-1 pathway
Gao, W., Yuan, Lm., Zhang, Y. et al.
Photochem Photobiol Sci 22, 135–146 (2023). https://doi.org/10.1007/s43630-022-00304-1
